Glycosylphosphatidylinositol anchors of membrane glycoproteins are binding determinants for the channel-forming toxin aerolysin.

The Journal of Biological Chemistry
D B DiepJ T Buckley

Abstract

Cells that are sensitive to the channel-forming toxin aerolysin contain surface glycoproteins that bind the toxin with high affinity. Here we show that a common feature of aerolysin receptors is the presence of a glycosylphosphatidylinositol anchor, and we present evidence that the anchor itself is an essential part of the toxin binding determinant. The glycosylphosphatidylinositol (GPI)-anchored T-lymphocyte protein Thy-1 is an example of a protein that acts as an aerolysin receptor. This protein retained its ability to bind aerolysin when it was expressed in Chinese hamster ovary cells, but could not bind the toxin when expressed in Escherichia coli, where the GPI anchor is absent. An unrelated GPI-anchored protein, the variant surface glycoprotein of trypanosomes, was shown to bind aerolysin with similar affinity to Thy-1, and this binding ability was significantly reduced when the anchor was removed chemically. Cathepsin D, a protein with no affinity for aerolysin, was converted to an aerolysin binding form when it was expressed as a GPI-anchored hybrid in COS cells. Not all GPI-anchored proteins bind aerolysin. In some cases this may be due to differences in the structure of the anchor itself. Thus the GPI-anchored protein...Continue Reading

References

Dec 1, 1975·The Biochemical Journal·M Letarte-MuirheadA F Williams
Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·F ZhangK Jacobson
Jan 15, 1991·The Biochemical Journal·M Horst, A Hasilik
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·W L RobertsT L Rosenberry
Mar 1, 1984·The Journal of Experimental Medicine·K C GunterE M Shevach
Jan 1, 1995·Annual Review of Immunology·R J Ulevitch, P S Tobias
Jun 26, 1995·Biochemical and Biophysical Research Communications·E Ogier-DenisP Codogno
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J D LeeR J Ulevitch
Mar 1, 1994·The Journal of Experimental Medicine·A O HueberH T He
Jan 1, 1993·Annual Review of Biochemistry·P T Englund
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·K R SolomonR W Finberg
Jan 1, 1996·Molecular Microbiology·M W ParkerJ T Buckley
Mar 14, 1997·The Journal of Biological Chemistry·P M RuddR A Dwek
Jun 1, 1997·The EMBO Journal·J RossjohnM W Parker

❮ Previous
Next ❯

Citations

Aug 10, 2000·Cytometry·M J Borowitz
Jun 15, 2006·Medical Microbiology and Immunology·Shenhui LangMichael Palmer
Nov 5, 2003·Experimental Cell Research·Dzung H Nguyen, Dennis D Taub
Oct 12, 2001·Toxicon : Official Journal of the International Society on Toxinology·M FivazF G van der Goot
Feb 7, 2001·Trends in Microbiology·L AbramiF G van der Goot
Nov 30, 2006·Biochemistry·Jody A Melton-WittRodney K Tweten
Jul 9, 2009·Journal of the American Chemical Society·Xueqing GuoZhongwu Guo
Jul 4, 2001·Nature Reviews. Molecular Cell Biology·G Schiavo, F G van der Goot
Jul 30, 2002·The EMBO Journal·Marc FivazF Gisou van der Goot
Mar 6, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Stephen D DertingerJames T MacGregor
Aug 10, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yukiko HashimotoAkihiro Harada
Dec 9, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Lucile PernotF Gisou van der Goot
Jan 24, 2003·Infection and Immunity·Laurence AbramiF Gisou van der Goot
Sep 20, 2002·Journal of Virology·Mireille GuyaderDidier Trono
May 9, 2002·Molecular and Cellular Biology·Federica SotgiaMichael P Lisanti
Nov 2, 2005·Molecular and Cellular Biology·Aki ManninenKai Simons
Feb 3, 2005·Blood·Rong HuRobert A Brodsky
Jul 30, 2005·Blood·Charles ParkerUNKNOWN International PNH Interest Group
Oct 17, 2009·Journal of Biomedical Science·Shaheen E LakhanAnanya De
Jun 21, 2011·PloS One·Pawel SzczesnyMarcin Grynberg
Aug 28, 2012·Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy·Günter Müller
Jul 27, 1999·Molecular Microbiology·D H NguyenJ E Hildreth
Nov 16, 2013·Trends in Microbiology·Ashley L DuMont, Victor J Torres
Oct 26, 2010·Journal of Biochemistry·Hiroyasu Shimada, Sakae Kitada
Sep 12, 2006·FEBS Letters·Núria Reig, F Gisou van der Goot
Oct 6, 1999·The Journal of Cell Biology·L Abrami, F G van Der Goot
Dec 8, 2015·Nature Reviews. Microbiology·Matteo Dal Peraro, F Gisou van der Goot

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.