Feb 1, 1985

Glycylprolyl dipeptidylaminopeptidase from Bacteroides gingivalis

Journal of Dental Research
Y AbikoH Takiguchi


Dipeptidyl aminopeptidase activity was found in the culture medium of Bacteroides gingivalis 381. The enzyme, hydrolyzing glycylprolyl-4-methylcoumaryl-7-amide, was purified 750-fold from culture medium by ammonium sulfate precipitation, Sephadex G-200 gel filtration, and DEAE Bio Gel A column chromatography. The molecular weight, determined by gel filtration, was approximately 160,000. The isoelectric point of the enzyme, estimated by isoelectric focusing using polyacrylamide disk gel electrophoresis, was about pH 6.2. The optimum pH of the enzyme was about 8.0, and the Km value was 0.05 mM. The enzyme activity was strongly inhibited by phenylmethylsulfonylfluoride and diisopropylfluorophosphate. The purified enzyme specifically cleaved glycylprolyl dipeptide from partially digested type I collagen.

Mentioned in this Paper

Chromatography, DEAE-Cellulose
Glycylprolyl protease
High Pressure Liquid Chromatography Procedure
Alveolar Pyorrhea
Molecular Sieve Chromatography
Hydrogen-Ion Concentration
Chemical Precipitation

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