Glycyrrhizin inhibits human parainfluenza virus type 2 replication by the inhibition of genome RNA, mRNA and protein syntheses

Drug Discoveries & Therapeutics
Kae Sakai-SuginoHiroshi Komada

Abstract

The effect of glycyrrhizin on the replication of human parainfluenza virus type 2 (hPIV-2) was examined. Cell fusion induced by hPIV-2 was inhibited by glycyrrhizin, and glycyrrhizin reduced the number of viruses released from the cells. Glycyrrhizin did not change cell morphology at 1 day of culture, but caused some damage at 4 days, as determined by the effect on actin microfilaments. However, it affected the cell viability at 1 day: about 20% of the cells were not alive by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay at 1 day of culture. Real-time polymerase chain reaction (PCR) and PCR showed that virus genome synthesis was largely inhibited. mRNA synthesis was also inhibited by glycyrrhizin. Viral protein synthesis was largely inhibited as observed by an indirect immunofluorescence study. Multinucleated giant cell formation was studied using a recombinant green fluorescence protein (GFP)-expressing hPIV-2 without matrix protein (rhPIV-2ΔMGFP). A few single cells with fluorescence were observed, but the formation of giant cells was completely blocked. Taken together, it was shown that viral genome, mRNA and protein syntheses, including F and HN proteins, were inhibited by glycyrrhizin, and conseq...Continue Reading

References

Jul 1, 1977·Journal of Virology·R W PelusoP W Choppin
May 10, 2005·Antiviral Research·Jindrich CinatlHans Wilhelm Doerr
Jan 9, 2009·Biomedical Research·Naomi TaodaHiroshi Komada
Jul 19, 2011·Journal of Translational Medicine·Usman A AshfaqSheikh Riazuddin
May 24, 2012·Virology Journal·Michele E HardyNicholas R Faunce
Jul 23, 2013·PloS One·Yoshihiro MatsumotoHideki Aizaki
Aug 27, 2014·Microbiology and Immunology·Sahoko KihiraHiroshi Komada

❮ Previous
Next ❯

Citations

Dec 17, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mohammed SikanderMeena Jaggi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Atherosclerosis Disease Progression

Atherosclerosis is the buildup of plaque on artery walls, causing stenosis which can eventually lead to clinically apparent cardiovascular disease. Find the latest research on atherosclerosis disease progression here.