Glyoxylate cycle gene ICL1 is essential for the metabolic flexibility and virulence of Candida glabrata.

Scientific Reports
Shu Yih ChewLeslie Thian Lung Than

Abstract

The human fungal pathogen Candida glabrata appears to utilise unique stealth, evasion and persistence strategies in subverting the onslaught of host immune response during systemic infection. However, macrophages actively deprive the intracellular fungal pathogen of glucose, and therefore alternative carbon sources probably support the growth and survival of engulfed C. glabrata. The present study aimed to investigate the role of the glyoxylate cycle gene ICL1 in alternative carbon utilisation and its importance for the virulence of C. glabrata. The data showed that disruption of ICL1 rendered C. glabrata unable to utilise acetate, ethanol or oleic acid. In addition, C. glabrata icl1∆ cells displayed significantly reduced biofilm growth in the presence of several alternative carbon sources. It was also found that ICL1 is crucial for the survival of C. glabrata in response to macrophage engulfment. Disruption of ICL1 also conferred a severe attenuation in the virulence of C. glabrata in the mouse model of invasive candidiasis. In conclusion, a functional glyoxylate cycle is essential for C. glabrata to utilise certain alternative carbon sources in vitro and to display full virulence in vivo. This reinforces the view that antifun...Continue Reading

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Citations

Feb 6, 2020·The Biochemical Journal·Maíra Pompeu MartinsNilce Maria Martinez-Rossi
Mar 19, 2020·Current Pharmaceutical Design·Kleber Santiago SilvaMaristela Pereira
May 15, 2019·International Journal of Molecular Sciences·Mónica GalochaMiguel C Teixeira
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Nov 17, 2020·Frontiers in Cell and Developmental Biology·Markus RudowitzWolfgang Schliebs
Jun 3, 2020·Journal of Proteomics·Sonia K Shishodia, Jata Shankar
Aug 27, 2021·Journal of Fungi·Yahaya HassanLeslie Thian Lung Than

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Methods Mentioned

BETA
PCR
enzyme-linked immunosorbent assay

Software Mentioned

GraphPad Prism
GraphPad

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