Glypican-1 and glycoprotein 2 bearing extracellular vesicles do not discern pancreatic cancer from benign pancreatic diseases

Oncotarget
Fabrice LucienHon S Leong

Abstract

Pancreatic ductal adenocarcinoma (PDAC) is a lethal disease that is clinically asymptomatic in its early stages of development. Non-invasive testing for pancreatic cancer biomarkers would significantly improve early detection and patient care. Extracellular vesicles (EVs) are circulating tumor fragments present in the blood and may express cancer specific biomarkers that would enable early detection of pancreatic cancer. We tested the utility of a blood test enumerating EVs positive for the pancreas-specific marker Glycoprotein 2 (GP2) and the putative pancreatic cancer marker Glypican-1 (GPC1) in patients with PDAC. Various levels of GPC1-positive and GP2/GPC1-positive EVs were detected in PDAC patients but were not significantly higher than benign pancreatic disease (BPD) patients. The sensitivity and specificity of the GPC1 EV test was 26.67% and 87.50% respectively, whereas the sensitivity and specificity for the GPC1+GP2 EV test was 23.33% and 90.00% respectively. Immunohistochemistry of GPC1 expression in a tissue microarray of PDAC and various controls also did not demonstrate specificity of GPC1 to PDAC. Hence, enumeration of GPC1-positive EVs, solely or in conjunction with GP2, was unable to effectively distinguish bet...Continue Reading

References

Jul 29, 1988·Biochemical and Biophysical Research Communications·D LeBel, M Beattie
Apr 11, 2003·Archives of Surgery·Carlos Fernández-del CastilloAndrew L Warshaw
Jan 21, 2004·Journal of Gastroenterology and Hepatology·Jee-Eun KimKyoo Wan Choi
Mar 27, 2004·Diseases of the Colon and Rectum·Shunji MoritaNobuhiro Shibata
Mar 31, 2004·Lancet·Donghui LiJames L Abbruzzese
Apr 20, 2004·Clinical and Applied Thrombosis/hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis·Kyung Soon Song, Hyun Kyung Kim
Jun 8, 2004·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Elizabeth A HollyManjushree Gautam
Feb 9, 2005·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·X G NiP Zhao
May 26, 2006·The American Journal of Pathology·Gui SuAndreas Friedl
Jul 1, 2008·Gynecologic Oncology·Douglas D Taylor, Cicek Gercel-Taylor
Mar 18, 2009·Clinical Lung Cancer·Guilherme RabinowitsGoetz H Kloecker
May 31, 2011·Lancet·Audrey VincentMichael Goggins
Oct 13, 2011·Nucleic Acids Research·Suresh MathivananRichard J Simpson
Dec 1, 2011·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Wigdan Al-SukhniSteven Gallinger
Mar 24, 2012·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Janusz Rak, Abhijit Guha
Oct 23, 2013·Cancer Research·Klaus Pantel, Catherine Alix-Panabières
May 9, 2014·Nature Biotechnology·Michael R Speicher, Klaus Pantel
Feb 26, 2016·Nature·Peter BaileySean M Grimmond
May 26, 2017·Science Translational Medicine·Katherine S YangRalph Weissleder
Sep 16, 2017·Frontiers in Cell and Developmental Biology·Joshua A WelshNicola A Englyst
Oct 27, 2017·Methods in Molecular Biology·John P Nolan, Erika Duggan

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Citations

Sep 23, 2020·Nature Reviews. Cancer·Andreas Möller, Richard J Lobb
Jul 30, 2019·Frontiers in Oncology·Sen WangBing Bai
Sep 26, 2020·Frontiers in Oncology·Lise NannanBertrand Brassart
Aug 7, 2020·Expert Review of Proteomics·Paula MeleadyMartin Clynes
Oct 27, 2020·Advanced Biosystems·Katherine S YangCesar M Castro
Apr 18, 2020·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Zijian YangErica L Carpenter
Apr 4, 2021·International Journal of Molecular Sciences·Randa G Hanna-SawiresRob A E M Tollenaar
Apr 2, 2021·Frontiers in Oncology·Yanan GaoKunyu Yang
Feb 23, 2021·Veterinary and Comparative Oncology·Seulji LeeWan Hee Kim
Sep 23, 2021·American Journal of Physiology. Cell Physiology·Jiajia Pan, Mitchell Ho

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Methods Mentioned

BETA
biopsies
biopsy
flow cytometry
flow
xenografts
xenograft
light scattering

Software Mentioned

Micro
nFC
GraphPad
A50
GraphPad Prism

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