Glypican-1-antibody-conjugated Gd-Au nanoclusters for FI/MRI dual-modal targeted detection of pancreatic cancer

International Journal of Nanomedicine
Xin HuangBingdi Chen

Abstract

Pancreatic cancer (PC) has a poor prognosis with high mortality, due to the lack of effective early diagnostic and prognostic tools. In order to target and diagnose PC, we developed a dual-modal imaging probe using Glypican-1 (GPC-1) antibody conjugated with Gd-Au nanoclusters (NCs; Gd-Au-NC-GPC-1). GPC-1 is a type of cell surface heparan sulfate proteoglycan, which is often highly expressed in PC. The probe was successfully prepared with a hydrodynamic diameter ranging from 13.5 to 24.4 nm. Spectral characteristics showed absorption at 280 nm and prominent emission at 650 nm. Confocal microscopic imaging showed effective detection of GPC-1 highly expressed PC cells by Gd-Au-NC-GPC-1, which was consistent with flow cytometry results. In vitro relaxivity characterization demonstrated that the r1 value of the probe was 17.722 s-1 mM-1 Gd, which was almost 4 times higher compared with that of Gd-diethylenetriaminepentacetate (DTPA; r1 value =4.6 s-1 mM-1 Gd). Gd-Au-NC-GPC-1 exhibited similar magnetic resonance (MR) signals when compared to Gd-DTPA even at lower Gd concentrations. Much higher MR signals were registered in PC cells (COLO-357) compared with normal cells (293T). Furthermore, Gd-Au-NC-GPC-1 could effectively detect PC ...Continue Reading

Citations

Feb 8, 2020·Dalton Transactions : an International Journal of Inorganic Chemistry·Claudine Herlan, Stefan Bräse
Feb 4, 2021·Bioengineering & Translational Medicine·Natacha JugniotRamasamy Paulmurugan
Dec 30, 2020·Cancers·Ruben D HouvastCornelis F M Sier
Jul 13, 2021·Frontiers in Oncology·Simone Furini, Chiara Falciani
Aug 1, 2021·Nanoscale Research Letters·Xin HuangBingdi Chen

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Methods Mentioned

BETA
fluorescence imaging
electron
transmission
atomic
X-ray
enzyme-linked immunosorbent assay
flow cytometry
NMR
confocal
confocal microscopy

Software Mentioned

Image
pro plus

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