PMID: 8604024Mar 1, 1996Paper

GM-CSF and IL-3 activities in schistosomal liver granulomas are controlled by stroma-associated heparan sulfate proteoglycans

Journal of Leukocyte Biology
M Alvarez-Silva, R Borojevic

Abstract

Connective tissue cells (myofibroblasts) from liver inflammatory granulomatous reactions to schistosome eggs are able to sustain a long-term proliferation of myeloid cells, both in vivo and in vitro. We have addressed the question of the molecular mechanisms involved in control of this extramedullar stroma-dependent production of inflammatory cells. Heparan sulfate proteoglycans (HSPGs) were purified from granuloma-derived connective tissue cells and bound to plastic or collagen substrate. Their ability to bind recombinant murine granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-3 (IL-3), to stimulate proliferation of the FDC-P1 myeloid cell lineage, and to modify growth factor activity was monitored. The specificity of this stroma cell-derived glycosaminoglycan interaction with the myeloid growth factors was analyzed by comparing other glycosaminoglycans and sulfated polysaccharides. HSPGs could act as an artificial myelopoietic stroma; they were both required and sufficient for binding and presenting GM-CSF and IL-3 in biologically active form. Moreover, they were able to mediate an increase in the specific growth-promoting activity of GM-CSF and IL-3. This was specific for stroma-derived heparan sulfa...Continue Reading

Citations

Dec 18, 2002·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Claudia B N Mendes de AguiarAndréa Gonçalves Trentin
Apr 26, 2003·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·D A PereiraR Borojevic
Apr 1, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Martin Götte
Dec 23, 2011·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Huafeng SunChunmao Han
Oct 12, 2001·American Journal of Physiology. Endocrinology and Metabolism·A G TrentinV Moura Neto
Aug 2, 2000·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·C L VituriP Borelli
Jul 20, 2005·The Journal of Biological Chemistry·Adriano SebollelaSérgio T Ferreira
Jun 8, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·L LuS Qian
Dec 17, 2008·Journal of Biochemistry·Satoko KishimotoMasayuki Ishihara
Jan 24, 2019·The Journal of Biological Chemistry·Khue G NguyenDavid A Zaharoff
Nov 9, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·R S Mummery, C C Rider
Jul 16, 2008·Immunology and Cell Biology·Deirdre R Coombe
Jul 15, 2017·Scientific Reports·Srinivas JayanthiDavid A Zaharoff
Oct 20, 2019·Journal of Controlled Release : Official Journal of the Controlled Release Society·Daniel HachimMolly M Stevens
Mar 7, 2021·International Journal of Molecular Sciences·Željka Sanader MaršićVlasta Bonačić-Kouteckỳ
Dec 8, 2006·Nutrition·Maristella A LandgrafZuleica B Fortes
Sep 12, 2006·Biochimica Et Biophysica Acta·Claudia M B AndradeFátima C R Guma
Jul 28, 2009·Biochemical and Biophysical Research Communications·Ana L ZiulkoskiFátima C R Guma

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.