GM-CSF Enhances Mobilization of Bone Marrow Mesenchymal Stem Cells via a CXCR4-Medicated Mechanism

Tissue Engineering and Regenerative Medicine
Jiyoung KimByung Hyune Choi

Abstract

This study was conducted to investigate the effect of granulocyte-macrophage colony-stimulating factor (GM-CSF) on the mobilization of mesenchymal stem cells (MSCs) from the bone marrow (BM) into the peripheral blood (PB) in rats. GM-CSF was administered subcutaneously to rats at 50 μg/kg body weight for 5 consecutive days. The BM and PB of rats were collected at 1, 3, and 5 days during the administration for analysis. Upon GM-CSF administration, the number of mononuclear cells increased rapidly at day 1 both in the BM and PB. This number decreased gradually over time in the BM to below the initial amount by day 5, but was maintained at a high level in the PB until day 5. The colony-forming unit-fibroblasts were increased in the PB by 10.3-fold at day 5 of GM-CSF administration, but decreased in the BM. Compared to GM-CSF, granulocyte-colony stimulating factor (G-CSF) stimulated lower levels of MSC mobilization from the BM to the PB. Immunohistochemical analysis revealed that GM-CSF induced a hypoxic and proteolytic microenvironment and increased C-X-C chemokine receptor type 4 (CXCR4) expression in the BM. GM-CSF added to BM MSCs in vitro dose-dependently increased CXCR4 expression and cell migration. G-CSF and stromal cell de...Continue Reading

References

Jan 1, 1995·Annual Review of Cell and Developmental Biology·S J MorrisonI L Weissman
Apr 2, 1999·Science·M F PittengerD R Marshak
Nov 1, 2000·Arthritis Research·N J ZvaiflerR N Maini
Apr 5, 2001·Nature·D OrlicP Anversa
May 31, 2001·The Journal of Cell Biology·S A KuznetsovP G Robey
Mar 18, 2003·Transplantation·Gordon D WuDonald V Cramer
Mar 2, 2006·JAMA : the Journal of the American Medical Association·Dietlind ZohlnhöferUNKNOWN REVIVAL-2 Investigators
Jun 17, 2006·Stem Cells·Gaël Y RochefortJorge Domenech
Aug 5, 2006·Journal of Cellular Biochemistry·Bruno NerviJohn F DiPersio
Jun 8, 2010·Cell Transplantation·Hiroshi YagiMartin L Yarmush
Jul 8, 2011·Stem Cells and Development·Marijke W MaijenburgCarlijn Voermans
Jun 16, 2012·Immunology Letters·Sara M Rankin
Oct 17, 2012·Blood Reviews·Ibraheem H Motabi, John F DiPersio
Mar 7, 2013·The Journal of Surgical Research·Changjiang HuGuangping Liang
Jul 26, 2013·American Journal of Physiology. Renal Physiology·Nanmei LiuJinyuan Zhang
Feb 18, 2015·Stem Cell Research & Therapy·Eduardo Branco de SousaDiego Pinheiro Aguiar
Sep 11, 2016·Stem Cell Research & Therapy·Sami G Almalki, Devendra K Agrawal
Mar 14, 2017·Tissue Engineering and Regenerative Medicine·Beatriz A Rodas-Junco, Claudia Villicaña
Jun 23, 2017·Tissue Engineering and Regenerative Medicine·Arantza InfanteIsabel Andia
Jul 27, 2017·Tissue Engineering and Regenerative Medicine·Mahsa SaniTahereh Talaei-Khozani

❮ Previous
Next ❯

Citations

Jun 6, 2020·Journal of Cardiovascular Pharmacology·Jin-Lian Shen, Xiao-Jie Xie
Sep 14, 2020·Stem Cell Reviews and Reports·Yogesh Kumar VermaGurudutta U Gangenahalli
May 16, 2019·Stem Cell Reviews and Reports·Anna M LenkiewiczMariusz Z Ratajczak

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

CSF & Lymphatic System

This feed focuses on Cerebral Spinal Fluid (CSF) and the lymphatic system. Discover the latest papers using imaging techniques to track CSF outflow into the lymphatic system in animal models.