PMID: 9663599Jul 15, 1998Paper

GM-CSF gene expression and protein production in human colorectal cancer cell lines and clinical tumor specimens

International Journal of Cancer. Journal International Du Cancer
M TrutmannGiulio C Spagnoli

Abstract

Granulocyte-macrophage colony stimulating factor (GM-CSF) gene expression and protein production were investigated in colorectal cancer cell lines and surgical specimens. In 6 of 6 established tumor lines, expression of the GM-CSF gene was observed by reverse transcription polymerase chain reaction (RT-PCR). Furthermore, for 2 of the lines, the cytokine was detectable in > or = 100 pg/ml amounts in culture supernatants by enzyme-linked immunosorbent assay tests. Addition of recombinant GM-CSF at doses ranging between 30 pg and 30 ng/ml did not appear to affect the proliferation of colorectal cancer cell lines as measured by 3H-thymidine incorporation. GM-CSF gene expression was then examined in surgical specimens by a densitometry-assisted, semiquantitative RT-PCR technique. In the 10 samples analyzed, significantly higher expression was detectable in tumors, as compared with autologous healthy mucosa sampled in the vicinity (2 cm) or at distance (10 cm) from the neoplastic focus. Immunohistochemistry studies performed on 13 specimens led to the identification of intracytoplasmic GM-CSF in tumor cells in 9 samples. In 6 of these, positivity of stromal fibroblasts and lymphocytes adjacent to the tumor was also observed. In contr...Continue Reading

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Citations

Jul 1, 2008·World Journal of Surgical Oncology·Hiroki TakahashiHiromitsu Takeyama
Dec 4, 2004·Clinical Chemistry and Laboratory Medicine : CCLM·Barbara Mroczko, Maciej Szmitkowski
Apr 22, 2005·Clinical Chemistry and Laboratory Medicine : CCLM·Barbara MroczkoGrazyna Jurkowska
Nov 27, 2010·Expert Review of Gastroenterology & Hepatology·Laia EgeaMartin F Kagnoff
Sep 16, 2006·Genes, Chromosomes & Cancer·Honor HugoRobert G Ramsay
Aug 18, 2010·International Journal of Cancer. Journal International Du Cancer·Giuseppe SconocchiaGiulio C Spagnoli
Mar 30, 2000·International Journal of Cancer. Journal International Du Cancer·G C SpagnoliM Heberer
Jul 1, 2005·Digestive Diseases and Sciences·Barbara MroczkoBogna Okulczyk
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Mar 27, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Gaëlle PicardaFranck Albert Halary
Jan 18, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Laia EgeaMartin F Kagnoff
Apr 17, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Christian A NebikerGiulio C Spagnoli

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