GM-CSF induces bone marrow precursors of NOD mice to skew into tolerogenic dendritic cells that protect against diabetes

Cellular Immunology
Simon GaudreauAbdelaziz Amrani

Abstract

We have reported that GM-CSF treatment of NOD mice suppressed diabetes by increasing the number of tolerogenic dendritic cells (tDCs) and Tregs in the periphery. Here, we have investigated whether GM-CSF acted on NOD bone marrow DCs precursors to skew their differentiation to tDCs. DCs were generated from the bone marrow of GM-CSF-treated (GM.BMDCs) and PBS-treated (PBS.BMDCs) NOD mice and were assessed for their ability to acquire tolerogenic properties. Upon LPS stimulation, GM.BMDCs became fully mature, expressed high levels of PD-L1 and produced more IL-10 and less IL-12p70 and IFN-gamma than PBS.BMDCs. In addition, LPS-stimulated GM.BMDCs possessed a reduced capacity to activate diabetogenic CD8(+) T cells in a PD-1/PD-L1-dependent manner. A single injection of LPS-stimulated GM.BMDCs in NOD mice resulted in long-term protection from diabetes, in contrast to LPS-stimulated PBS.BMDCs. Our results showed that GM-CSF-treatment acted on bone marrow precursors to skew their differentiation into tDCs that protected NOD mice against diabetes.

References

Dec 31, 1997·The Journal of Experimental Medicine·J VerdaguerP Santamaria
Apr 3, 2001·The Journal of Experimental Medicine·S Y TsengH Tsuchiya
Jul 23, 2003·The Journal of Experimental Medicine·Sayuri YamazakiRalph M Steinman
Jul 24, 2003·Nature Reviews. Immunology·Carlos Ardavín
Sep 30, 2003·European Journal of Immunology·Spencer C LiangArlene H Sharpe
May 11, 2006·Blood·Sergio RutellaGiuseppe Leone
Jul 14, 2007·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Indira GuleriaMohamed H Sayegh
Jun 14, 2008·Nature Reviews. Immunology·John A Hamilton
Feb 17, 2009·Human Immunology·Kerstin SteinbrinkHelmut Jonuleit

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Citations

Sep 25, 2012·Trends in Immunology·John A Hamilton, Adrian Achuthan
Aug 19, 2015·Scientific Reports·Young Mee YoonBenjamin G Keselowsky
Dec 1, 2011·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Jermaine I G CowardSanjay Popat
Jan 3, 2012·Frontiers in Neuroendocrinology·Rory D Spence, Rhonda R Voskuhl
Apr 7, 2015·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Jamal S LewisBenjamin G Keselowsky
Oct 24, 2017·Cellular & Molecular Immunology·Weidong ZhaoBin Wang
Jun 15, 2011·Current Opinion in Organ Transplantation·Alison J LeishmanPaul J Fairchild
Apr 20, 2018·Cancer Immunology Research·Girija GoyalGlenn Dranoff

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