GM-CSF/IL-3/IL-5 receptor common beta chain (CD131) expression as a biomarker of antigen-stimulated CD8+ T cells.

Journal of Translational Medicine
Silvia SelleriFrancesco M Marincola

Abstract

Upon Ag-activation cytotoxic T cells (CTLs) produce IFN-gamma GM-CSF and TNF-alpha, which deliver simultaneously pro-apoptotic and pro-inflammatory signals to the surrounding microenvironment. Whether this secretion affects in an autocrine loop the CTLs themselves is unknown. Here, we compared the transcriptional profile of Ag-activated, Flu-specific CTL stimulated with the FLU M1:58-66 peptide to that of convivial CTLs expanded in vitro in the same culture. PBMCs from 6 HLA-A*0201 expressing donors were expanded for 7 days in culture following Flu M1:58-66 stimulation in the presence of 300 IU/ml of interleukin-2 and than sorted by high speed sorting to high purity CD8+ expressing T cells gated according to FluM1:58-66 tetrameric human leukocyte antigen complexes expression. Ag-activated CTLs displayed higher levels of IFN-gamma, GM-CSF (CSF2) and GM-CSF/IL-3/IL-5 receptor common beta- chain (CD131) but lacked completely expression of IFN-gamma receptor-II and IFN-stimulated genes (ISGs). This observation suggested that Ag-activated CTLs in preparation for the release of IFN-gamma and GM-CSF shield themselves from the potentially apoptotic effects of the former entrusting their survival to GM-SCF. In vitro phenotyping confirme...Continue Reading

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Citations

Oct 16, 2010·Journal of Immunotherapy·Takashi InozumeJames C Yang
Jun 19, 2009·Journal of Translational Medicine·Hideaki TaharaFrancesco M Marincola
Aug 31, 2011·Journal of Translational Medicine·Maria Libera AsciertoFrancesco M Marincola
May 7, 2008·Trends in Immunology·Ena WangFrancesco M Marincola
Oct 14, 2011·Journal of Translational Medicine·Tara L SpiveyDavide Bedognetti
May 5, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Patrick Williams, Jacques Galipeau
May 20, 2014·Molecular Cancer Research : MCR·Ping LiZeev Estrov

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Datasets Mentioned

BETA
GM-CSF

Methods Mentioned

BETA
dissection
leukapheresis
flow-cytometry
FACS
flow cytometry

Software Mentioned

Tree View
Cluster
ArrayTools
BRB
ArrayTool
Database for Annotation , Visualization and Integrated Discove...
BRB Array

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