GM3-enriched microdomain involved in cell adhesion and signal transduction through carbohydrate-carbohydrate interaction in mouse melanoma B16 cells.

The Journal of Biological Chemistry
K IwabuchiS Hakomori

Abstract

Mouse melanoma B16 cells are characterized by the predominant presence of ganglioside GM3 and adhere to lactosylceramide- or Gg3-coated plates through interaction of GM3 with lactosylceramide or Gg3, whereby not only adhesion but also spreading and enhancement of cell motility occur (Kojima, N., Hakomori, S. (1991) J. Biol. Chem. 266, 17552-17558). We now report that the adhesion process is based essentially on a glycosphingolipid-enriched microdomain (GEM) at the B16 cell surface, since >90% of GM3 present in the original cells is found in GEM, and GEM is also enriched in several signal transducer molecules, e.g. c-Src, Ras, Rho, and focal adhesion kinase (FAK). GEM was isolated as a low density membranous fraction by homogenization of B16 cells in lysis buffer under two different conditions (i.e. buffer containing 1% Triton X-100, or hypertonic sodium carbonate without detergent), followed by sucrose density gradient centrifugation. A close association of GM3 with c-Src, Rho, and FAK was indicated by co-immunoprecipitation of GM3 present in GEM by anti-GM3 monoclonal antibody DH2, followed by Western blotting with antibodies directed to these transducer molecules. The following data indicate that GEM is a structural and funct...Continue Reading

References

Dec 31, 1991·Biochemical and Biophysical Research Communications·K HandaS Hakomori
Feb 21, 1992·Cell·K G RothbergR G Anderson
Apr 20, 1990·Cell·A Ullrich, J Schlessinger
Jul 27, 1990·Cell·K Simons, A Wandinger-Ness
Aug 23, 1990·Nature·J DownwardD A Cantrell
Aug 23, 1988·Biochemistry·K Simons, G van Meer
Aug 24, 1983·Biochimica Et Biophysica Acta·T W TillackW W Young
Oct 24, 1995·Proceedings of the National Academy of Sciences of the United States of America·E J SmartR G Anderson
May 23, 1995·Proceedings of the National Academy of Sciences of the United States of America·T MutohN Fujiki
Sep 8, 1995·Science·R G Parton, K Simons
Jan 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·L J YangR L Schnaar
Apr 26, 1996·The Journal of Biological Chemistry·P LiuR G Anderson
May 17, 1996·The Journal of Biological Chemistry·C MineoR G Anderson
Jun 5, 1997·Nature·K Simons, E Ikonen
Sep 25, 1955·The Journal of Biophysical and Biochemical Cytology·E YAMADA

❮ Previous
Next ❯

Citations

Jul 9, 1999·Developmental Dynamics : an Official Publication of the American Association of Anatomists·K M Murphy, S E Zalik
Jan 7, 2005·Chemistry : a European Journal·Injae ShinMyung-ryul Lee
Mar 5, 2013·Archivum Immunologiae Et Therapiae Experimentalis·Hitoshi NakayamaKazuhisa Iwabuchi
Jun 16, 2007·Cancer Immunology, Immunotherapy : CII·Diego O CrociO Graciela Scharovsky
Apr 25, 2007·Apoptosis : an International Journal on Programmed Cell Death·Walter MalorniMaurizio Sorice
Aug 4, 1999·Immunology Today·V HorejsíH Stockinger
Jan 13, 2000·Biophysical Chemistry·K Kasahara, Y Sanai
Sep 9, 2000·Current Opinion in Cell Biology·G Pande
Sep 9, 2000·Current Opinion in Cell Biology·L Ossowski, J A Aguirre-Ghiso
Jul 27, 2001·Biochimica Et Biophysica Acta·M Masserini, D Ravasi
Aug 15, 2002·Biochimica Et Biophysica Acta·F MartiniL Lenti
Oct 21, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Xuguang ChenAssem G Ziady
Apr 22, 2009·Proceedings of the National Academy of Sciences of the United States of America·Feng GuanSen-itiroh Hakomori
Aug 20, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Feng GuanSen-Itiroh Hakomori
Dec 17, 2002·Current Opinion in Hematology·Senitiroh Hakomori
Apr 18, 2006·Biochemistry. Biokhimii︠a︡·E V Dyatlovitskaya, A G Kandyba
Jun 30, 2007·BMC Developmental Biology·Susanna AmadioCinzia Volonté
Jan 2, 2009·Journal of Cell Science·Naoki IchikawaEri Arikawa-Hirasawa
May 3, 2008·Biological & Pharmaceutical Bulletin·Tasuku KawanoKazuo Nitta
Apr 6, 2007·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Hideyoshi Higashi
Jun 30, 2000·Biochemical and Biophysical Research Communications·A KazuiS Hakomori
Jul 17, 2001·Biochemical and Biophysical Research Communications·K HandaS I Hakomori
Jan 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Sen-itiroh Hakomori Si
Jun 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Alka A VyasRonald L Schnaar
Nov 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·Yutaka MiuraSenitiroh Hakomori
Nov 15, 2006·The Journal of Cell Biology·Thomas B Nicholson, Clifford P Stanners

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.