GMDS knockdown impairs cell proliferation and survival in human lung adenocarcinoma

BMC Cancer
Xing WeiHong Wang

Abstract

Lung adenocarcinoma is the most common type of lung cancer and one of the most lethal and prevalent cancers. Aberrant glycosylation was common and essential in tumorigenesis, with fucosylation as one of the most common types disrupted in cancers. However, it is still unknown whether genes involved in fucosylation are important for lung adenocarcinoma development and process. GMDS is involved in cellular fucosylation. Here we examined GMDS expression level at both mRNA and protein level in lung adenocarcinoma. The impact of GMDS knockdown on lung adenocarcinoma in vitro and in vivo was investigated. Transcriptome changes with GMDS knockdown in lung adenocarcinoma cells were also examined to provide insights into related molecular mechanisms. GMDS expression is significantly upregulated in lung adenocarcinoma at both mRNA and protein levels. Lentivirus-mediated shRNA strategy inhibited GMDS expression efficiently in human lung adenocarcinoma cells A549 and H1299, and GMDS knockdown impaired cell proliferation, colony formation ability, induced cell cycle arrest, and apoptosis in both cell lines. Furthermore, GMDS knockdown inhibited tumorigenesis in a xenograft mice model of lung adenocarcinoma. Microarray analysis explored the G...Continue Reading

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Citations

May 19, 2019·G3 : Genes - Genomes - Genetics·Barbara KlotzManfred Schartl
Jan 9, 2019·Frontiers in Oncology·Liyuan JiaJihong Cui
Dec 29, 2020·Frontiers in Oncology·Isabel Castro-PiedrasKevin Pruitt
Jul 1, 2019·G3 : Genes - Genomes - Genetics·Barbara KlotzManfred Schartl

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Datasets Mentioned

BETA
GMDS
GSE104123
GSE31210

Methods Mentioned

BETA
RNAseq
PCR
Protein Assay
electrophoresis
xenograft
in vitro transcription
fluorescence-activated cell sorting
flow cytometry
ubiquitination
glycosylation

Software Mentioned

Cellomics ArrayScan VTI
GeneChip
GraphPad Prism
Cellomics ArrayScan
Ingenuity Pathway Analysis ( IPA )

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