PMID: 7539640Jan 1, 1995Paper

GmN56, a novel nodule-specific cDNA from soybean root nodules encodes a protein homologous to isopropylmalate synthase and homocitrate synthase

Molecular Plant-microbe Interactions : MPMI
Hiroshi Kouchi, S Hata

Abstract

We have isolated a novel nodule-specific cDNA clone, GmN56, from soybean root nodules. The expression of GmN56 was induced almost concomitantly with the onset of nitrogen fixation, together with leghemoglobin and other late nodulin genes. In situ hybridization studies demonstrated the localization of GmN56 mRNA in the bacterial infected cells of mature nodules. The predicted amino acid sequence of the GmN56 protein exhibits significant homology to those of LeuA (isopropylmalate synthase) of several microorganisms and NifV (putative homocitrate synthase) of nitrogen-fixing bacteria, suggesting that GmN56 encodes an enzyme catalyzing a reaction involving acetyl-CoA and alpha-keto acid as substrates.

Citations

Jul 28, 2010·Plant & Cell Physiology·Hiroshi KouchiMasayoshi Kawaguchi
Apr 9, 2008·Molecular Plant-microbe Interactions : MPMI·Laurent BrechenmacherGary Stacey
Oct 24, 2000·Molecular Plant-microbe Interactions : MPMI·R B DayG Stacey
May 25, 2002·Molecular Plant-microbe Interactions : MPMI·Emmanouil FlemetakisPanagiotis Katinakis
Apr 23, 2010·The Plant Journal : for Cell and Molecular Biology·Marc LibaultGary Stacey

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