GmRIN4 protein family members function nonredundantly in soybean race-specific resistance against Pseudomonas syringae.

The New Phytologist
Devarshi SeloteAardra Kachroo

Abstract

The Pseudomonas syringae effector AvrB interacts with four related soybean (Glycine max) proteins (GmRIN4a-d), three (GmRIN4b, c, d) of which also interact with the cognate resistance (R) protein, Rpg1-b. Here, we investigated the specific requirements for the GmRIN4 proteins in R-mediated resistance and examined the mechanism of Rpg1-b activation. Using virus-induced gene silencing, we show that only GmRIN4a and b are required for Rpg1-b-mediated resistance. In planta binding assays show that GmRIN4a can associate with Rpg1-b indirectly via GmRIN4b. Pathogen-delivered AvrB induces the phosphorylation of GmRIN4b alone, and prevents interactions between GmRIN4b and Rpg1-b or GmRIN4a. Consistent with this result, a phosphomimic derivative of GmRIN4b (pm4b) fails to bind Rpg1-b and GmRIN4a. Conversely, a phosphodeficient derivative of GmRIN4b (pd4b) continues to bind the R protein and GmRIN4a, in the presence of AvrB. Coexpression of Rpg1-b with pm4b, but not GmRIN4b or pd4b, induces cell death and ion leakage in the heterologous Nicotiana benthamiana. Our data suggest that the AvrB-induced phosphorylation of GmRIN4b, and the subsequent inhibition of interaction among GmRIN4b, GmRIN4a and Rpg1-b, might activate the R protein. Furt...Continue Reading

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Citations

Jan 1, 2014·The New Phytologist·Devarshi SeloteAardra Kachroo
Apr 3, 2015·Briefings in Functional Genomics·Jian-Zhong LiuSteven A Whitham
Jan 28, 2015·Molecular Plant-microbe Interactions : MPMI·Andrew R RussellRoger W Innes
Jul 1, 2016·Annual Review of Phytopathology·Steven A WhithamTarek Hewezi
Nov 13, 2018·Molecular Plant-microbe Interactions : MPMI·Tania Y ToruñoDavid Mackey
Jan 4, 2021·Planta·Guangdong ZhaoXingqi Guo
Dec 10, 2020·International Journal of Molecular Sciences·Yee-Shan KuHon-Ming Lam
Nov 21, 2020·The Plant Genome·Chantal E McCabe, Michelle A Graham

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