Goat AKAP12: Indel Mutation Detection, Association Analysis With Litter Size and Alternative Splicing Variant Expression.

Frontiers in Genetics
Zihong KangHaiyu Zhao

Abstract

A-kinase anchoring protein 12 (AKAP12) plays key roles in male germ cells and female ovarian granulosa cells, whereas its influence on livestock litter size remains unclear. Herein we detected the genetic variants of AKAP12 gene and their effects on litter size as well as alternative splicing variants expression in Shaanbei white cashmere (SBWC) goats, aiming at exploring theoretical basis for goat molecular breeding. We identified two Insertion/deletions (Indels) (7- and 13-bp) within the AKAP12 gene. Statistical analyses demonstrated that the 13-bp indel mutation in the 3' UTR was significantly associated with litter size (n = 1,019), and the carriers with DD genotypes presented lower litter sizes compared with other carriers (P < 0.01). Bioinformatics analysis predicted that this 13-bp deletion sequence could bind to the seed region of miR-181, which has been documented to suppress porcine reproductive and respiratory syndrome virus (PRRSV) infection by targeting PRRSV receptor CD163 and affect the pig litter size. Therefore, luciferase assay for this 13-bp indel binding with miRNA-181 was performed, and the luciferase activity of pcDNA-miR-181-13bp-Deletion-allele vector was significantly lower than that of the pcDNA-miR-18...Continue Reading

References

Feb 1, 1974·Genetics·M Nei, A K Roychoudhury
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Aug 24, 2007·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Bertrand C Y Collard, David J Mackill
Mar 28, 2009·Molecular Endocrinology·Bonnie J DerooKenneth S Korach
Dec 12, 2018·Journal of Cellular Physiology·Rasoul AbdollahzadehJavad Tavakkoly-Bazzaz
Oct 19, 2019·Molecular Cell·Jernej Ule, Benjamin J Blencowe
Jun 24, 2020·Journal of the American Heart Association·Hanan Qasim, Bradley K McConnell
Dec 3, 2020·International Journal of Molecular Sciences·Ji Hae SeoKen Arai
Mar 7, 2021·International Journal of Molecular Sciences·Izarbe Aísa-MarínGemma Marfany

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Software Mentioned

SHEsis
RNAhybrid

Related Concepts

Related Feeds

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.