Goat and buffalo milk fat globule membranes exhibit better effects at inducing apoptosis and reduction the viability of HT-29 cells

Scientific Reports
Xiaoxi JiShaobo Zhou

Abstract

Bovine milk fat globule membrane (MFGM) has shown many health benefits, however, there has not been much study on non-cattle MFGMs. The purpose of this study was to compare the anti-proliferation effects and investigate the mechanisms of MFGMs from bovine, goat, buffalo, yak and camel milk in HT-29 cells. Results showed that protein content in MFGM of yak milk is the highest among five MFGM. All MFGMs reduced cellular viability which was in agreement with cell morphology and apoptosis. However, the number of cells in S-phase from 24 h to 72 h was increased significantly by treatment with goat, buffalo and bovine MFGMs (100 μg/mL), but not yak and camel. All MFGMs treatment significantly reduced the mitochondrial membrane potential (with an order of goat > buffalo > bovine > camel > yak) and Bcl-2 expression, but increased the expression of both Bax and Caspase-3. Taken together, the results indicate that all MFGMs, especially goat and buffalo MFGMs, showed better effects at inducing apoptosis and reduction the viability of HT-29 cells. The mechanism might be arresting the cell cycle at S phase, depolarization of mitochondrial membrane potential, down-regulation of Bcl-2 expression and increase of Bax and Caspase-3 expression.

References

Oct 1, 1972·Analytical Biochemistry·P J Geiger, S P Bessman
Jun 15, 1995·European Journal of Biochemistry·V L SpitsbergR C Gorewit
Feb 7, 1997·Cell·S Nagata
Aug 15, 1997·The Biochemical Journal·G M Cohen
Jun 2, 1998·Biochimica Et Biophysica Acta·S Cory, J M Adams
Jul 8, 1998·Medicine·L C Brody, B B Biesecker
Feb 26, 2000·The Journal of Investigative Dermatology·G LiV C Ho
Feb 15, 2001·Cell Death and Differentiation·Y Tsujimoto, S Shimizu
Mar 10, 2001·Cold Spring Harbor Symposia on Quantitative Biology·S J KorsmeyerS Zinkel
Apr 18, 2001·Cell Research·X Q Fan, Y J Guo
Jan 10, 2002·Biochimica Et Biophysica Acta·Cécile VissacDominique J Bernard-Gallon
Apr 25, 2002·Proceedings of the National Academy of Sciences of the United States of America·Catherine CommunalRoger J Hajjar
Jun 6, 2002·European Journal of Biochemistry·Lone T PallesenJan T Rasmussen
Jul 9, 2004·Drug Metabolism Reviews·Roger Harrison
Aug 24, 2004·Infection and Immunity·Hannah M MartinRoger Harrison
Jun 16, 2005·Journal of Dairy Science·V L Spitsberg
Jan 4, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Masahiro YaoYoshinobu Kubota
Feb 29, 2008·BMB Reports·Seon-Yong Jeong, Dai-Wu Seol
Apr 22, 2008·Methods in Molecular Biology·Ezgi TasdemirGuido Kroemer
Oct 29, 2008·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Jae-Yong KimKwon-Il Seo
Mar 27, 2009·The American Journal of Drug and Alcohol Abuse·C E GreenJ P Carbonari
Feb 23, 2010·Journal of Dairy Science·C CeboP Martin
Dec 15, 2010·Journal of Proteomics·Salvatore PisanuMaria Filippa Addis
May 28, 2011·Thyroid : Official Journal of the American Thyroid Association·Kee-Hyun NamCheong Soo Park
Dec 24, 2011·Molecular BioSystems·Stefano SpertinoMaria Cavaletto
Apr 3, 2012·International Journal of Pharmaceutics·Shengpeng WangYitao Wang
Sep 18, 2012·Journal of Dairy Science·A TellezV Delcenserie
Oct 17, 2012·Food & Function·Romina ZanabriaMilena Corredig
Mar 29, 2014·Trends in Molecular Medicine·Sritama Nath, Pinku Mukherjee
Apr 17, 2014·Stem Cells·James S HaleJustin D Lathia
Apr 5, 2016·Mediators of Inflammation·Ji Hye KimJae Youl Cho

❮ Previous
Next ❯

Methods Mentioned

BETA
glycosylation
flow cytometry
fluorescence microscopy
transmission electron microscopy

Software Mentioned

Image J
SPSS

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

Indian Journal of Biochemistry & Biophysics
M K Bhavadasan, N C Ganguli
Biochimica Et Biophysica Acta
S Patton, T W Keenan
Biochimica Et Biophysica Acta
R M DowbenD E Philpott
The International Journal of Biochemistry
C J HofmannW N Eigel
© 2022 Meta ULC. All rights reserved