Goblet cells and mucin related gene expression in mice infected with Eimeria papillata

TheScientificWorldJournal
Mohamed Abdel Monam DkhilSaleh Al-Quraishy

Abstract

Coccidiosis causes considerable economic loss in the poultry industry. The current study aimed to investigate the response of goblet cells as well as the induced tissue damage during Eimeria papillata infection. Mice were infected with sporulated E. papillata oocytes. On day 5 postinfection, the fecal output was determined. Also, the jejunum was prepared for the histological, histochemical, and molecular studies. Our results revealed that the intestinal coccidian infection with E. papillata induced a marked goblet cell hypoplasia and depleted mucus secretion. Also, the infection was able to alter the jejunal architecture and increased the apoptotic cells inside the villi. In addition, the real-time PCR results indicated that the inflammatory cytokines: TNF- α , iNOS, IFN- γ , and IL-1 β , were significantly upregulated. In contrast, the mRNA expression patterns of IL-6 in response to E. papillata infection did not differ significantly between control and infected mice. Moreover, the mRNA expression of TLR4 was significantly upregulated, whereas the expression of MUC2 is significantly downregulated upon infection. Further studies are required to understand the regulatory mechanisms of goblet cells related genes.

References

Jan 1, 1986·Scandinavian Journal of Gastroenterology. Supplement·A AllenL A Sellers
Mar 19, 1996·Proceedings of the National Academy of Sciences of the United States of America·D L Vaux, A Strasser
Jun 8, 2001·The American Journal of Clinical Nutrition·B Deplancke, H R Gaskins
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Sep 25, 2003·Nature Reviews. Molecular Cell Biology·Douglas Taupin, Daniel K Podolsky
Apr 5, 2005·The Journal of Veterinary Medical Science·Muchammad YunusAdrian L Smith
Jul 13, 2006·Gastroenterology·Maria Van der SluisAlexandra W C Einerhand
Aug 13, 2010·Current Gastroenterology Reports·Young S Kim, Samuel B Ho
Nov 26, 2010·Experimental Parasitology·Mohamed DkhilSaleh Al-Quraishy
Feb 9, 2011·Parasitology Research·Saleh Al-QuraishyMohamed Abdel Monam Dkhil
May 25, 2011·Journal of Biomedical Informatics·Harsh DweepNorbert Gretz
Sep 14, 2012·Parasitology Research·Mohamed A DkhilDenis Delic
Jan 30, 2013·The American Journal of Pathology·Vanessa Kissoon-SinghKris Chadee

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Citations

Dec 15, 2015·Frontiers in Veterinary Science·Juxing ChenJeffery Escobar
Jul 17, 2016·BMC Complementary and Alternative Medicine·Murad A MubarakiSaleh Al-Quraishy
May 18, 2017·Scandinavian Journal of Immunology·M Abdel-LatifH Abdel-Tawab
Jun 12, 2018·Critical Reviews in Food Science and Nutrition·Amlan Kumar PatraJörg Rudolf Aschenbach
Nov 12, 2019·Journal of Animal Physiology and Animal Nutrition·Liqing HuangZhaofei Xia
Nov 14, 2019·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Saleh Al-QuraishyMohamed Abdel Monam Dkhil
Mar 3, 2020·Frontiers in Veterinary Science·Adrien W D SanchesElizabeth Santin

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