May 18, 2016

Goggatomy: a Method for Opening Small Cuticular Compartments in Arthropods for Physiological Experiments

BioRxiv : the Preprint Server for Biology
Alan R KayDaniel F Eberl

Abstract

Most sense organs of arthropods are ensconced in small exoskeletal compartments that hinder direct access to plasma membranes. We have developed a method for exposing live sensory and supporting cells in such structures. The technique uses a viscous light cured resin to embed and support the structure, which is then sliced with a sharp blade. We term the procedure a goggatomy, from the Khoisan word for a bug, gogga. To demonstrate the utility of the method we show that it can be used to expose the auditory chordotonal organs in the second antennal segment and the olfactory receptor neurons in the third antennal segment of Drosophila melanogaster, preserving the transduction machinery. The procedure can also be used on other small arthropods, like mites, Daphnia, mosquitoes, wasps and ants to expose a variety of cells.

  • References
  • Citations

References

  • We're still populating references for this paper, please check back later.
  • References
  • Citations

Citations

  • This paper may not have been cited yet.

Mentioned in this Paper

Mites
Daphnia antigen
Transduction
Compartment of Cell
Organ
Embedding
AS resin
Sense Organs
Structure
Anatomical Compartments

About this Paper

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.

Related Papers

Current Biology : CB
Ryan G Kavlie, Jörg T Albert
Cold Spring Harbor Symposia on Quantitative Biology
C A G WIERSMA
© 2020 Meta ULC. All rights reserved