Gold manno-glyconanoparticles for intervening in HIV gp120 carbohydrate-mediated processes

Methods in Enzymology
Paolo Di GianvincenzoSoledad Penadés

Abstract

After nearly three decades since the discovery of human immunodeficiency virus (HIV) (1983), no effective vaccine or microbicide is available, and the virus continues to infect millions of people worldwide each year. HIV antiretroviral drugs reduce the death rate and improve the quality of life in infected patients, but they are not able to completely remove HIV from the body. The glycoprotein gp120, part of the envelope glycoprotein (Env) of HIV, is responsible for virus entry and infection of host cells. High-mannose type glycans that decorate gp120 are involved in different carbohydrate-mediated HIV binding. We have demonstrated that oligomannoside-coated gold nanoparticles (manno-GNPs) are able to interfere with HIV high-mannose glycan-mediated processes. In this chapter, we describe the methods for the preparation and characterization of manno-GNPs and the experiments performed by means of SPR and STD-NMR techniques to evaluate the ability of manno-GNPs to inhibit 2G12 antibody binding to gp120. The antibody 2G12-mediated HIV neutralization and the lectin DC-SIGN-mediated HIV trans-infection in cellular systems are also described.

Citations

May 23, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sónia Alexandra Correia Carabineiro
Aug 8, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sabine SzuneritsRabah Boukherroub
Jul 6, 2014·Beilstein Journal of Organic Chemistry·Fabrizio ChiodoSoledad Penadés
Jun 26, 2020·Frontiers in Chemistry·Sundas Bahar YaqoobMohammad Rashid
Jul 16, 2021·Carbohydrate Research·Rituparna Das, Balaram Mukhopadhyay
Jul 16, 2020·ACS Nano·Giacomo ReinaAlberto Bianco

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