Gold Nanocluster Containing Polymeric Microcapsules for Intracellular Ratiometric Fluorescence Biosensing

ACS Omega
Aniket BiswasMichael J McShane

Abstract

A new approach to sensing and imaging hydrogen peroxide (H2O2) was developed using microcapsule-based dual-emission ratiometric luminescent biosensors. Bovine serum albumin-capped gold nanoclusters (BSA-AuNCs) sensitive to H2O2 were coencapsulated with insensitive FluoSpheres (FSs) within polymeric capsules fabricated via the layer-by-layer method. Under single-wavelength excitation, the microcapsule-based biosensors exhibited emission bands at ∼516 and ∼682 nm resulting from the FSs and BSA-AuNCs, respectively. The polyelectrolyte multilayers lining the microcapsules were effective in protecting BSA-AuNCs from the degradation catalyzed by proteases (chymotrypsin, trypsin, papain, and proteinase K) and subsequent luminescent quenching, overcoming a key limitation of prior BSA-AuNC-based sensing systems. The luminescent response of the sensors was also found to be independent of local changes in pH (5-9). Quenching of the AuNCs in the presence of H2O2 enabled the spectroscopic quantification and imaging of changes in H2O2 concentration from 0 to 1 mM. The microcapsule sensors were easily phagocytized by murine macrophage cells (RAW 264.7), were effective as intracellular H2O2 imaging probes, and were successfully used to detect ...Continue Reading

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Citations

Nov 27, 2019·Advanced Healthcare Materials·Nesma El-SayedMarc Schneider
Feb 28, 2020·Analytical and Bioanalytical Chemistry·Louis Van der MeerenAndre G Skirtach
Sep 10, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Nesma El-Sayed, Marc Schneider
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Nov 10, 2020·Frontiers in Bioengineering and Biotechnology·Jia TangZhenghua Tang
Jul 21, 2021·Chemical Reviews·W Russ AlgarHyungki Kim
Oct 9, 2021·Nanoscale·Bárbara CasteleiroJosé Paulo Sequeira Farinha
Jan 20, 2022·Analytical Methods : Advancing Methods and Applications·Hua-Rui NanXiao-Hong Cao

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Methods Mentioned

BETA
biosensing
confocal microscopy
PMA

Software Mentioned

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