Gold nanoparticle-based colorimetric assay for the direct detection of cancerous cells

Analytical Chemistry
Colin D MedleyWeihong Tan

Abstract

Early and accurate detection of cancer often requires time-consuming techniques and expensive instrumentation. To address these limitations, we developed a colorimetric assay for the direct detection of diseased cells. The assay uses aptamer-conjugated gold nanoparticles to combine the selectivity and affinity of aptamers and the spectroscopic advantages of gold nanoparticles to allow for the sensitive detection of cancer cells. Samples with the target cells present exhibited a distinct color change while nontarget samples did not elicit any change in color. The assay also showed excellent sensitivity with both the naked eye and based on absorbance measurements. In addition, the assay was able to differentiate between different types of target and control cells based on the aptamer used in the assay indicating the wide applicability of the assay for diseased cell detection. On the basis of these qualities, aptamer-conjugated gold nanoparticles could become a powerful tool for point of care diagnostics.

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Citations

May 19, 2010·Analytical and Bioanalytical Chemistry·Ying PuWeihong Tan
Aug 10, 2010·Analytical and Bioanalytical Chemistry·Maureen A WallingJason R E Shepard
Nov 6, 2010·Analytical and Bioanalytical Chemistry·Ana María Coto-GarcíaAlfredo Sanz-Medel
Dec 17, 2010·Analytical and Bioanalytical Chemistry·Boonchoy Soontornworajit, Yong Wang
Dec 22, 2010·Analytical and Bioanalytical Chemistry·Briza Pérez-López, Arben Merkoçi
May 23, 2012·Analytical and Bioanalytical Chemistry·Diana VilelaAlberto Escarpa
Aug 25, 2009·Bioprocess and Biosystems Engineering·Yoon-Jin KimMan Bock Gu
Jan 27, 2012·Applied Biochemistry and Biotechnology·Xiu-Mei JiangChun-Ying Chen
Aug 21, 2013·Biosensors & Bioelectronics·Subash C B GopinathKoichi Awazu
Nov 13, 2009·Analytical Chemistry·Guodong LiuLingwen Zeng
Jun 2, 2012·ACS Applied Materials & Interfaces·Mohammed I ShukoorWeihong Tan
Oct 1, 2013·ACS Applied Materials & Interfaces·Cuifeng JiangQing-Hua Xu
Sep 16, 2009·Accounts of Chemical Research·Xiaohong Fang, Weihong Tan
Mar 21, 2013·Chemical Reviews·Weihong TanJianhui Jiang
Apr 26, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Nishi BhattJuewen Liu
Sep 13, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Nicholas M AdamsDavid W Wright
Jun 3, 2011·Molecular Pharmaceutics·Pinal C PatelChad A Mirkin
Mar 24, 2010·Nano Letters·Joshua I CutlerChad A Mirkin
Jul 29, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Amy Yan, Andrew Ellington
Jul 10, 2009·Chemical Society Reviews·Elodie Boisselier, Didier Astruc
Nov 6, 2008·Chemical Communications : Chem Comm·Dong-Kwon LimJwa-Min Nam
Apr 1, 2009·Chemical Communications : Chem Comm·Tingting JiangBengang Xing
Mar 4, 2011·Proceedings of the National Academy of Sciences of the United States of America·Hui ShiBing Zhou
Jan 16, 2014·Interface Focus·Emir YasunWeihong Tan
Jul 20, 2010·Annual Review of Analytical Chemistry·Eun Jeong ChoAndrew D Ellington
Aug 25, 2010·Journal of Nanobiotechnology·Johanna G WalterStephan Barcikowski
Apr 1, 2009·International Journal of Molecular Sciences·Maureen A WallingJason R E Shepard
Apr 11, 2012·International Journal of Molecular Sciences·Mao YeWeihong Tan
Jan 1, 2010·Sensors·Young-Eun ChoiJoon Won Park
May 16, 2013·Sensors·Nicola S FracchiollaAgostino Cortelezzi
Jul 27, 2010·Bioanalysis·Yunfei ZhangWeihong Tan

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