Gold nanoparticle-based immunoassay by using non-stripping chemiluminescence detection

The Analyst
Chun-Feng DuanHua Cui

Abstract

A novel microplate-compatible chemiluminescence (CL) immunoassay has been developed for the determination of human immunoglobulin G (IgG) based on the luminol-AgNO(3)-gold nanoparticles CL system. Polystyrene microtiter plates were used for both immunoreactions and CL measurements. The primary antibody, goat-anti-human IgG, was first immobilized on polystyrene microwells. Then the antigen (human IgG) and the gold-labeled second antibody were connected to the microwells successively to form a sandwich-type immunocomplex. The gold label could trigger the reaction between luminol and AgNO(3), accompanied by light emission. Under the optimized conditions, the CL intensity of the system was linear with the logarithm of the concentration of human IgG in the range from 25 to 5000 ng mL(-1), with a detection limit of 12.8 ng mL(-1) ( approximately 80 pM) at a signal to noise ratio of three (S/N = 3). Compared with other reported CL immunoassay method based on gold labels, the proposed CL protocol avoids a strict stripping procedure or difficult to control synthesis processes, making the method more simple, time-saving and easily automated. The present CL method is promising for the determination of clinically important bioactive analytes.

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Citations

May 17, 2011·Analytical and Bioanalytical Chemistry·Cui Liu, Baoxin Li
Jun 18, 2009·Chemical Communications : Chem Comm·Chun-Feng Duan, Hua Cui
Apr 23, 2011·Chemical Communications : Chem Comm·Sheng CaiXueji Zhang
Jun 9, 2014·Analytical and Bioanalytical Chemistry·Na LiHua Cui
May 12, 2009·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Aiping FanJianzhong Lu
Aug 25, 2011·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Honglan QiChengxiao Zhang
Jun 7, 2011·Biosensors & Bioelectronics·Chinnasamy ThiruppathirajaMuthukaruppan Alagar
Aug 3, 2011·Luminescence : the Journal of Biological and Chemical Luminescence·Shifeng LiXianwen Wei
Nov 28, 2014·Luminescence : the Journal of Biological and Chemical Luminescence·Abdolraouf Samadi-Maybodi, Reza Akhoondi
May 20, 2014·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Jing LuoBaoxin Li
Feb 24, 2015·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Bozorgmehr MaddahMehdi Rahimi-Nasrabadi
Aug 31, 2016·Scientific Reports·Maryam Shahrajabian, M Reza Hormozi-Nezhad

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