Gold nanoparticle-enhanced multiplexed imaging surface plasmon resonance (iSPR) detection of Fusarium mycotoxins in wheat

Biosensors & Bioelectronics
Md Zakir Hossain, Chris M Maragos

Abstract

A rapid, sensitive and multiplexed imaging surface plasmon resonance (iSPR) biosensor assay was developed and validated for three Fusarium toxins, deoxynivalenol (DON), zearalenone (ZEA) and T-2 toxin. The iSPR assay was based on a competitive inhibition format with secondary antibodies (Ab2) conjugated to gold nanoparticles (AuNPs) used as a signal amplification tag. Signal was amplified nearly 25-fold for DON, 90-fold for ZEA and 12-fold for T-2 toxin assay using Ab2-AuNPs. Analyses, including steps to regenerate the sensor, took 17.5min. The antigen coated sensor chip was used for more than 46 cycles without affecting signal intensity (< 12%). Matrix matched calibration curves were used to determine Fusarium toxins in wheat. The mean recoveries ranged from 87% to 103% with relative standard deviations of repeatability of less than 5%. The limits of detection were 15µg/kg for DON, 24µg/kg for ZEA and 12µg/kg for T-2 toxin. This provided sufficient sensitivity to monitor contamination of these mycotoxins in wheat in accordance with European Commission (EC) limits. Cut off levels for all three Fusarium toxins were validated using blank wheat and wheat spiked either at the EC regulated levels (100µg/kg for ZEA and T-2 toxin) or ...Continue Reading

References

Oct 1, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Iuliana E SendroiuRobert M Corn
Apr 13, 2011·Analytical and Bioanalytical Chemistry·Denis DorokhinMichel W F Nielen
Apr 12, 2012·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·C M Maragos
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Sep 4, 2016·Analytica Chimica Acta·Aleksandra KarczmarczykKarl-Heinz Feller

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Citations

Apr 27, 2019·Analytical and Bioanalytical Chemistry·Zakir HossainChris M Maragos
Jul 28, 2020·Critical Reviews in Analytical Chemistry·Mustafa Oguzhan CaglayanZafer Üstündağ
Apr 25, 2020·Foods·Sofia AgriopoulouTheodoros Varzakas
May 27, 2021·Analytical Methods : Advancing Methods and Applications·Ting GuoShuo Wang
Aug 7, 2021·Toxins·Krisztina Majer-BaranyiAndrás Székács
Aug 21, 2020·Journal of Agricultural and Food Chemistry·Bin Wang, Bosoon Park
Oct 4, 2021·Comprehensive Reviews in Food Science and Food Safety·Weizheng WangSundaram Gunasekaran

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