Gold nanoparticles-conjugated quercetin induces apoptosis via inhibition of EGFR/PI3K/Akt-mediated pathway in breast cancer cell lines (MCF-7 and MDA-MB-231)

Cell Biochemistry and Function
Solaimuthu BalakrishnanJ Arunakaran

Abstract

Epidermal growth factor plays a major role in breast cancer cell proliferation, survival, and metastasis. Quercetin, a bioactive flavonoid, is shown to exhibit anticarcinogenic effects against various cancers including breast cancer. Hence, the present study was designed to evaluate the effects of gold nanoparticles-conjugated quercetin (AuNPs-Qu-5) in MCF-7 and MDA-MB-231 breast cancer cell lines. Borohydride reduced AuNPs were synthesized and conjugated with quercetin to yield AuNPs-Qu-5. Both were thoroughly characterized by several physicochemical techniques, and their cytotoxic effects were assessed by MTT assay. Apoptotic studies such as DAPI, AO/EtBr dual staining, and annexin V-FITC staining were performed. AuNPs and AuNPs-Qu-5 were spherical with crystalline nature, and the size of particles range from 3.0 to 4.5 nm. AuNPs-Qu-5 exhibited lower IC50 value compared to free Qu. There was a considerable increase in apoptotic population with increased nuclear condensation seen upon treatment with AuNPs-Qu-5. To delineate the molecular mechanism behind its apoptotic role, we analysed the proteins involved in apoptosis and epidermal growth factor receptor (EGFR)-mediated PI3K/Akt/GSK-3β signalling by immunoblotting and immuno...Continue Reading

References

Mar 8, 1995·Biochemical and Biophysical Research Communications·R L SinghalG Weber
Oct 18, 1996·Cell·E S AlnemriJ Yuan
Jan 23, 2002·Cell Death and Differentiation·Guy S Salvesen
Mar 8, 2002·Cellular Signalling·Karleen M Nicholson, Neil G Anderson
Aug 7, 2004·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Maria Fernanda Cury-BoaventuraRui Curi
Oct 4, 2005·The Journal of Clinical Investigation·Inna N LavrikPeter H Krammer
May 3, 2006·The Prostate·Celeste L KremerRaymond B Nagle
Aug 1, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Takuro NiidomeYasuro Niidome
Apr 25, 2007·Drug Development and Industrial Pharmacy·K Vijaya SriA Annapurna
Sep 3, 2008·Chemical Society Reviews·Ralph A SperlingWolfgang J Parak
Sep 24, 2009·Journal of the American Chemical Society·Jutaek NamSungjee Kim
Nov 20, 2009·The FEBS Journal·Tetsuya Mitsudomi, Yasushi Yatabe
Jan 2, 2010·Analytical Chemistry·Adriano AmbrosiArben Merkoçi
Jan 22, 2010·Recent Patents on Anti-cancer Drug Discovery·Hai L LiuMeng X Xie
Feb 16, 2010·Pharmacology & Therapeutics·Vasilis P AndroutsopoulosDemetrios A Spandidos
Mar 17, 2010·Journal of the American Chemical Society·Sarah D BrownNial J Wheate
Dec 16, 2010·Nature Reviews. Molecular Cell Biology·Roberto ZoncuDavid M Sabatini
Mar 2, 2011·Dalton Transactions : an International Journal of Inorganic Chemistry·Thomas D SchladtWolfgang Tremel
Mar 16, 2011·IEEE Transactions on Bio-medical Engineering·Dustin E KruseKatherine W Ferrara
May 28, 2011·Breast Cancer Research and Treatment·Kevin KalinskyMary Ellen Moynahan
Aug 6, 2011·Journal of the American Chemical Society·Aleksandar VacicMark A Reed
Sep 21, 2011·International Journal of Cancer. Journal International Du Cancer·Vicki L J WhitehallBarbara A Leggett
Nov 18, 2011·Colloids and Surfaces. B, Biointerfaces·Guobin DingXuezhong Zhang
Jan 13, 2012·Nanotechnology·Arshad S SayyadPulickel M Ajayan
Feb 9, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Haisheng ZhangXingbin Yang
Apr 17, 2012·Cell·Mathieu Laplante, David M Sabatini
Feb 26, 2013·Colloids and Surfaces. B, Biointerfaces·Moorthy GaneshkumarLonchin Suguna
May 7, 2013·European Journal of Medicinal Chemistry·Ying-Chao DuanHong-Min Liu
Nov 14, 2013·Experimental and Therapeutic Medicine·Xiao-Hui DengFeng-Jin Zheng
Feb 8, 2014·Theranostics·Sudip MukherjeeChitta Ranjan Patra
May 14, 2014·International Journal of Molecular Sciences·Alle MadhusudhanSurya S Singh
Jul 12, 2014·BioMed Research International·Mohamed HassanNoriaki Sakuragi
Dec 3, 2014·Chemico-biological Interactions·Sindhu KondathRama Rajaram
Dec 2, 2015·Experimental and Therapeutic Medicine·Ming-Xin RenHai-Yan Song

❮ Previous
Next ❯

Citations

Jul 25, 2018·Phytotherapy Research : PTR·Abdur RaufMohammad S Mubarak
Sep 7, 2018·Journal of Cellular Biochemistry·Ammad Ahmad FarooqiBaojun Xu
Mar 14, 2019·Nanoscale·Hadi Ranji-BurachalooGreg G Qiao
Dec 1, 2018·Journal of Cellular Physiology·Raedeh SaraeiAli Hassanzadeh
Apr 25, 2019·Medicina·Firoozeh NiazvandAli Khodadadi
May 9, 2019·Biomolecules·Dharambir KashyapSardul Singh Sandhu
May 17, 2019·Biomaterials Science·Susheel Kumar NethiSudip Mukherjee
Jan 16, 2020·International Journal of Molecular Sciences·Anubhab MukherjeeSudip Mukherjee
Nov 23, 2019·Inflammopharmacology·Neda KasiriHossein Motedayyen
Jun 11, 2020·Archives of Physiology and Biochemistry·Masoud NajafiMilad Ashrafizadeh
May 30, 2020·Naunyn-Schmiedeberg's Archives of Pharmacology·Uma Priya MohanSelvaraj Kunjiappan
Dec 26, 2018·Evidence-based Complementary and Alternative Medicine : ECAM·Benjiao GongHuishan Zhao
Jul 10, 2019·Anti-cancer Agents in Medicinal Chemistry·Manjula Vinayak, Akhilendra K Maurya
Dec 21, 2018·International Journal of Molecular Sciences·Muhammad OvaisSudip Mukherjee
May 1, 2019·Cancers·Anubhab MukherjeeSudip Mukherjee
Aug 6, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Danielly C Ferraz da CostaEliane Fialho
Nov 27, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Qi-Yao WeiAndy T Y Lau
Aug 3, 2019·Seminars in Cancer Biology·Haroon KhanMohammad Amjad Kamal
Aug 28, 2019·Seminars in Cancer Biology·Paola AielloIlaria Peluso
Jan 23, 2021·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Duoyi ZhaoXuesi Chen
Oct 21, 2018·Free Radical Biology & Medicine·Wentong LiWeifen Zhang
Mar 13, 2021·Frontiers in Chemistry·Ajita JindalAftab Alam
Apr 20, 2021·Frontiers in Oncology·María Zenaida Saavedra-LeosMacrina Beatriz Silva-Cázares
Oct 15, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Bingyan YangYu Zhang
Jun 22, 2021·Food & Function·Xinlong ZangXuehong Chen
Jul 2, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Soudeh Ghafouri-FardSeyed Abdulmajid Ayatollahi

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Cancer Cell Invasion: Nanomedicine

Nanomedicine is a promising alternative for cancer detection and therapy that utilizes nanoparticles, such as liposomes. Nanoparticles can potentially target cancer cell invasion and metastasis. Discover the latest research on Cancer Cell Invasion: Nanomedicine here.

Breast Invasive Carcinoma (Keystone)

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.

Breast Invasive Carcinoma

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.