Gold nanoparticles do not induce myotube cytotoxicity but increase the susceptibility to cell death

Toxicology in Vitro : an International Journal Published in Association with BIBRA
Paulo Emílio Corrêa LeiteJosé Mauro Granjeiro

Abstract

Gold nanoparticles (AuNP) have been widely used for many applications, including as biological carriers. A better understanding concerning AuNP safety on muscle cells is crucial, since it could be a potential tool in the nanomedicine field. Here, we describe the impact of polyethylene glycol-coated gold nanoparticles (PEG-AuNP) interaction with differentiated skeletal muscle C2C12 cells on cell viability, mitochondria function, cell signaling related to survival, cytokine levels and susceptibility to apoptosis. Intracellular localization of 4.5 nm PEG-AuNP diameter size was evidenced by STEM-in-SEM in myotube cells. Methods for cytotoxicity analysis showed that PEG-AuNP did not affect cell viability, but intracellular ATP levels and mitochondrial membrane potential increased. Phosphorylation of ERK was not altered but p-AKT levels reduced (p<0.01). Pre-treatment of cells with PEG-AuNP followed by staurosporine induction increased the caspases-3/7 activity. Indeed, cytokines analysis revealed a sharp increase of IFN-γ and TGF-β1 levels after PEG-AuNP treatment, suggesting that inflammatory and fibrotic phenotypes process were activated. These data demonstrate that PEG-AuNP affect the myotube physiology leading these cells to be ...Continue Reading

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Citations

Jun 28, 2015·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Paulo Emílio Corrêa LeiteJosé Mauro Granjeiro
Jun 9, 2016·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Andrew R CollinsMaria Dusinska
Jul 22, 2020·Advanced Healthcare Materials·Amy Corbin FarrAntonios G Mikos
May 28, 2019·Journal of Cellular Biochemistry·Talíria Silva LopesPaulo Emílio Corrêa Leite
Dec 16, 2020·ACS Biomaterials Science & Engineering·Suliman KhanMojtaba Falahati
Nov 9, 2021·Nanomedicine·Franco TacchiClaudio Cabello-Verrugio

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