Gold Nanoparticles Enhance EGFR Inhibition and Irradiation Effects in Head and Neck Squamous Carcinoma Cells.

BioMed Research International
Masahiko KashinMasaya Akashi

Abstract

Cetuximab, an epidermal growth factor receptor inhibitor (EI), is currently the only targeted molecular therapy used in combination with radiotherapy for head and neck squamous cell carcinoma (HNSCC). Gold nanoparticles (AuNPs) are expected to enhance radiotherapy effects in cancers. To investigate whether AuNPs combined with AG1478, an EI, enhanced irradiation effects on HNSCC cells, we first examined AG1478 adsorption on AuNP surfaces, using surface-enhanced Raman scattering, which indicated an adsorption equilibrium of AG1478 to AuNPs. We then used transmission electron microscopy to find internalization rates of AuNP alone and AuNP+AG1478; we found that intracellular uptake of AuNP alone and AuNP+AG1478 did not significantly differ. We compared cell numbers, proliferation, apoptosis, and migration between control cells and those treated with or without 60 nm AuNP (1.0 nM), AG1478 (0.5 μM), and irradiation (4 Gy). We found that AuNP+AG1478 inhibited proliferation more than AG1478 alone; the combination of irradiation+AuNP+AG1478 significantly reduced total cell numbers compared with the combination of irradiation+AuNP; AuNP+AG1478 increased apoptotic reaction to irradiation; the combinations of AuNP+AG1478 and irradiation+Au...Continue Reading

References

Jan 1, 1997·Radiation Oncology Investigations·M T SheridanC E Mothersill
Jun 13, 1998·Journal of the National Cancer Institute·J Rubin GrandisD J Tweardy
Feb 10, 2006·The New England Journal of Medicine·James A BonnerK Kian Ang
Jul 26, 2008·Nature Nanotechnology·Wen JiangWarren C W Chan
Sep 12, 2008·The New England Journal of Medicine·Jan B VermorkenRicardo Hitt
Apr 16, 2011·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Feng YangDeliang Fu
Jan 1, 2010·Journal of Nanoparticle Research : an Interdisciplinary Forum for Nanoscale Science and Technology·Christian VilliersPatrice Marche
Feb 14, 2012·Colloids and Surfaces. B, Biointerfaces·Hui LiuXiangyang Shi
Mar 27, 2012·Colloids and Surfaces. B, Biointerfaces·Prachi JoshiSurinder P Singh
Nov 1, 2012·Biotechnology Advances·Anil KumarXing-Jie Liang
Sep 26, 2013·Colloids and Surfaces. B, Biointerfaces·Andreia Fonseca de FariaOswaldo Luiz Alves
Nov 2, 2013·Colloids and Surfaces. B, Biointerfaces·Ranjeet Kumar MohantyAsit Baran Mandal
Apr 30, 2014·Journal of Colloid and Interface Science·Anh Thu Ngoc LamSang-Woo Joo
Sep 14, 2014·Colloids and Surfaces. B, Biointerfaces·Anh Thu Ngoc LamSang-Woo Joo
Apr 9, 2017·Journal of Photochemistry and Photobiology. B, Biology·Meihua ZhangWoo Kyung Moon
Nov 14, 2017·Head & Neck·Erez Shmuel DavidiRachela Popovtzer
Jun 11, 2018·Nanomedicine : Nanotechnology, Biology, and Medicine·Tetsushi KubotaToshiyoshi Fujiwara
Feb 1, 2019·Experimental & Molecular Medicine·Hyung Kwon ByeonJaemoon Yang

❮ Previous
Next ❯

Methods Mentioned

BETA
surface plasmon resonance
X-ray

Software Mentioned

R Development Core Team
BZ
Keyence
R
H4M

Related Concepts

Related Feeds

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.