Gold nanoparticles enhance immune responses in mice against recombinant classical swine fever virus E2 protein

Biotechnology Letters
Yafei LiGaiping Zhang

Abstract

To create a novel subunit vaccine that used AuNPs as carriers to enhance immune responses in mice against recombinant classical swine fever virus E2 protein (CSFV E2). Gold nanoparticles (AuNPs) were successfully coupled to the E2 protein and formed stable particle complexes called E2 conjugated AuNPs (E2-AuNPs). In vitro studies have shown that the E2-AuNPs complex has the same immunogenicity as the E2 protein, and AuNPs can promote the phagocytosis of the E2 protein by antigen-presenting cells (APCs). In vivo results of BALB/c mice showed that the antibody levels, lymphocyte proliferation index, IFN-γ and IL-10 cytokines induced by E2-AuNPs were relatively higher than those of E2 or AuNPs group. This finding demonstrated the potential of using AuNPs as a carrier to enhance the body's immune response for developing CSFV subunit vaccines. This model also contributes to the development of other flavivirus subunit vaccines, such as hepatitis C virus (HCV) and bovine viral diarrhea virus (BVDV).

References

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Datasets Mentioned

BETA
AF531433

Methods Mentioned

BETA
transmission
dynamic light scattering
protein assay
Assay
enzyme-linked immunosorbent assay
ELISA
fluorescence microscopy
infrared spectroscopy

Software Mentioned

GraphPad Prism
GraphPad Prism5
GraphPad

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