Gold nanoparticles for tracking bacteria clearance by regulated irrigation and negative pressure-assisted wound therapy

Nanomedicine
Menachem MotieiMoris Topaz

Abstract

Regulated negative pressure-assisted wound therapy is a fundamental, nonpharmaceutical technology for acute and chronically infected wounds, yet bacterial clearance and biofilm buildup remain a challenge for healing. Regulated irrigation combined with negative pressure (RI-NPT) is emerging as an alternative therapeutic strategy for reducing bacterial load. Here, we analyzed RI-NPT hydrokinetics and efficacy of bacterial load reduction in wounds. Escherichia coli were loaded with gold nanoparticles, quantified by flame atomic absorption spectroscopy. Computed tomography (CT) imaging tracked bacterial distribution over time in a low-flow rat wound model. Bacterial load was quantified using a novel CT ruler. Flame atomic absorption spectroscopy showed loading of 1.7 × 103 ± 0.2 gold nanoparticles/cell. CT tracking revealed that while regulated negative pressure-assisted wound therapy reduced bacterial load to a limited extent (5%), RI-NPT significantly increased bacterial outflow and clearance (by 45%). This nanotechnology-based approach demonstrates that RI-NPT is essential for reducing bacterial load and, thus, for promoting wound healing.

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Methods Mentioned

BETA
transmission electron microscopy
scanning electron microscopy
Flame Atomic Absorption Spectroscopy
RGBA

Software Mentioned

NRecon
SkyScan CT - Volume ( CTVol )
SkyScan CT - Voxel ( CTVox )

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