Gold nanoparticles with chitosan, N-acylated chitosan, and chitosan oligosaccharide as DNA carriers

Nanoscale Research Letters
Paulina Abrica-GonzálezMiguel Ibáñez-Hernández

Abstract

Currently, gold nanoparticles have found applications in engineering and medical sciences, taking advantage from their properties and characteristics. Surface plasmon resonance, for instance, is one of the main features for optical applications and other physical properties, like high density, that represents the key for cellular uptake. Among other applications, in the medical field, some diseases may be treated by using gene therapy, including monogenetic or polygenetic disorders and infections. Gene adding, suppression, or substitution is one of the many options for genetic manipulation. This work explores an alternative non-viral method for gene transfer by using gold nanoparticles functionalized with organic polymers; two routes of synthesis were used: one of them with sodium borohydride as reducing agent and the other one with chitosan oligosaccharide as reducing and stabilizing agent. Gold nanoparticles conjugated with chitosan, acylated chitosan and chitosan oligosaccharide, were used to evaluate transfection efficiency of plasmid DNA into cell culture (HEK-293). Physical and chemical properties of gold nanocomposites were characterized by using UV-Vis Spectroscopy, ξ-potential, and transmission electron microscopy. Fur...Continue Reading

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Citations

Dec 25, 2019·Antimicrobial Agents and Chemotherapy·Alaa RiezkSimon L Croft
Mar 11, 2020·Pharmaceutics·Catarina Roma-RodriguesAlexandra R Fernandes
Jan 21, 2021·Acta Pharmacologica Sinica·Yun-Qiu MiaoYong Gan
Feb 7, 2021·Scientific Reports·Célia AribMarc Lamy de la Chapelle
Mar 9, 2021·Wiley Interdisciplinary Reviews. RNA·Mingyuan ZouJian Xu
Jun 13, 2021·Seminars in Cancer Biology·Ali Talha KhalilHari Parsad Devkota

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Methods Mentioned

BETA
transfection
acylation
surface plasmon resonance
electrophoresis
fluorescence microscopy
transfections

Software Mentioned

Gen5
Matlab®
Image J Tool

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