Golga5 is dispensable for mouse embryonic development and postnatal survival

Genesis : the Journal of Genetics and Development
Lynessa J McGeeYu Lan

Abstract

Golgins are a family of coiled-coil proteins located at the cytoplasmic surface of the Golgi apparatus and have been implicated in maintaining Golgi structural integrity through acting as tethering factors for retrograde vesicle transport. Whereas knockdown of several individual golgins in cultured cells caused Golgi fragmentation and disruption of vesicle trafficking, analysis of mutant mouse models lacking individual golgins have discovered tissue-specific developmental functions. Recently, homozygous loss of function of GOLGA2, of which previous in vitro studies suggested an essential role in maintenance of Golgi structure and in mitosis, has been associated with a neuromuscular disorder in human patients, which highlights the need for understanding the developmental roles of the golgins in vivo. We report here generation of Golga5-deficient mice using CRISPR/Cas9-mediated genome editing. Although knockdown studies in cultured cells have implicated Golga5 in maintenance of Golgi organization, we show that Golga5 is not required for mouse embryonic development, postnatal survival, or fertility. Moreover, whereas Golga5 is structurally closely related to Golgb1, we show that inactivation of Golga5 does not enhance the severity...Continue Reading

References

Dec 1, 1995·The Journal of Cell Biology·N NakamuraG Warren
May 26, 1999·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Y ZhangY Chen
Jul 27, 2002·Annual Review of Cell and Developmental Biology·James Shorter, Graham Warren
Jan 18, 2005·Autoimmunity Reviews·Kazuhisa NozawaEdward K L Chan
Dec 30, 2008·PLoS Genetics·John A FollitGregory J Pazour
Jun 11, 2009·Seminars in Cell & Developmental Biology·Irene Barinaga-Rementeria Ramirez, Martin Lowe
Jan 22, 2010·The New England Journal of Medicine·Patrick SmitsDavid R Beier
Mar 2, 2010·Circulation. Cardiovascular Imaging·Karl DegenhardtJonathan A Epstein
Mar 10, 2010·The Journal of Cell Biology·Christine Sütterlin, Antonino Colanzi
Oct 30, 2013·Proceedings of the National Academy of Sciences of the United States of America·Han LiuRulang Jiang
Jan 9, 2016·PLoS Genetics·Jingyue XuRulang Jiang
Mar 15, 2016·Trends in Cell Biology·Alison K Gillingham, Sean Munro
Dec 29, 2016·Proceedings of the National Academy of Sciences of the United States of America·Chunyi LiuShilai Bao
Jan 6, 2017·Cell Death & Disease·Feng HanFei Gao

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Citations

Nov 3, 2017·Journal of Cell Science·Nicola L StevensonDavid J Stephens
Jun 12, 2019·Journal of Cell Science·Javier Coy-VergaraBlanche Schwappach
Sep 12, 2019·Journal of Dental Research·Y LanR Jiang
May 28, 2019·Frontiers in Cell and Developmental Biology·Mario O CaracciMaría-Paz Marzolo
Jul 19, 2019·Frontiers in Cell and Developmental Biology·Martin Lowe
Sep 24, 2019·Frontiers in Cell and Developmental Biology·Ayano SatohKunihiko Nishino

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