Golgi 58K-like protein in pollens and pollen tubes of Lilium davidii

Science in China. Series C, Life Sciences
Y Li, L Yan

Abstract

In animal cells, Golgi apparatus is located near the microtubule organizing center (MTOC) and its position is determined partly by 58K protein. By sodium dodecyl-sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immuno-blotting methods, a 58K-like protein has been found in pollen grains and pollen tubes of Lilium davidii. Its molecular weight is very similar to that of the 58K protein of animal cells. By immunofluorescence labeling, under a confocal laser scanning microscope (CLSM), the animal 58K antibody revealed a punctate staining in pollen grains and pollen tubes, which is consistent with the distribution of Golgi apparatus in plant cells. In addition, immuno-gold labeling and transmission electron microscopy showed that the 58K-like protein bound mainly to the membrane of vesicles-like structure near Golgi apparatus. This is the first demonstration of the 58K-like protein in plant cells.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jul 25, 1998·The Journal of Biological Chemistry·A M Bashour, G S Bloom
Aug 8, 1998·Trends in Cell Biology·M G Farquhar, G E Palade
Aug 28, 1998·Current Opinion in Cell Biology·G Warren, V Malhotra
Sep 29, 1998·The Plant Journal : for Cell and Molecular Biology·P BoevinkC Hawes
Jul 1, 1992·Plant Physiology·X Liu, L F Yen

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Citations

Oct 6, 2010·Protoplasma·Giampiero Cai, Mauro Cresti

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