Golgi-Resident GTPase Rab30 Promotes the Biogenesis of Pathogen-Containing Autophagosomes

PloS One
Seiichiro OdaIchiro Nakagawa

Abstract

Autophagy acts as a host-defense system against pathogenic microorganisms such as Group A Streptococcus (GAS). Autophagy is a membrane-mediated degradation system that is regulated by intracellular membrane trafficking regulators, including small GTPase Rab proteins. Here, we identified Rab30 as a novel regulator of GAS-containing autophagosome-like vacuoles (GcAVs). We found that Rab30, a Golgi-resident Rab, was recruited to GcAVs in response to autophagy induction by GAS infection in epithelial cells. Rab30 recruitment was dependent upon its GTPase activity. In addition, the knockdown of Rab30 expression significantly reduced GcAV formation efficiency and impaired intracellular GAS degradation. Rab30 normally functions to maintain the structural integrity of the Golgi complex, but GcAV formation occurred even when the Golgi apparatus was disrupted. Although Rab30 also colocalized with a starvation-induced autophagosome, Rab30 was not required for autophagosome formation during starvation. These results suggest that Rab30 mediates autophagy against GAS independently of its normal cellular role in the structural maintenance of the Golgi apparatus, and autophagosome biogenesis during bacterial infection involves specific Rab GTP...Continue Reading

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Citations

Apr 6, 2017·Progress in Neurobiology·Sandrine PassemardVincent El Ghouzzi
Jan 15, 2017·Biochimica Et Biophysica Acta. Molecular Cell Research·Lu ZhangYuQi Guo
Dec 15, 2017·Small GTPases·Marcellus J Banworth, Guangpu Li
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Nov 15, 2018·Cellular Microbiology·Ching-Yu LinIchiro Nakagawa
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Jan 21, 2017·Bioscience Reports·Li-Qiang ZhengCheng-Xin Li
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Dec 19, 2019·Frontiers in Cellular and Infection Microbiology·Hirotaka TohIchiro Nakagawa
Jun 11, 2019·Autophagy·Hirotaka TohIchiro Nakagawa
Nov 30, 2018·Frontiers in Cellular and Infection Microbiology·Chihiro AikawaIchiro Nakagawa
Jan 1, 2019·Advanced Biosystems·Fujing Wei, Yixiang Duan
Jan 12, 2021·Frontiers in Cellular and Infection Microbiology·Ching-Yu LinIchiro Nakagawa
Dec 29, 2020·Experimental Cell Research·Khalisah L ZulkefliPaul A Gleeson
Feb 10, 2021·Cell & Bioscience·Qingchun LuLing Yang
Jul 22, 2021·The Journal of Cell Biology·Gabrielle LarocqueStephen J Royle
Mar 18, 2019·Developmental Neuroscience·Sowmyalakshmi RasikaVincent El Ghouzzi

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Datasets Mentioned

BETA
GM130

Methods Mentioned

BETA
confocal microscopy
transfections
PCR
GTPase
GTPases

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