Golgi staining-like retrograde labeling of brain circuits using rabies virus: Focus onto the striatonigral neurons.

Journal of Neuroscience Methods
Pascal SalinPatrice Coulon

Abstract

The introduction of viral transneuronal tracers in the toolbox of neural tract-tracing methods has been an important addition in the field of connectomics for deciphering circuit-level architecture of the nervous system. One of the added values of viral compared to conventional retrograde tracers, in particular of rabies virus, is to provide a Golgi staining-like view of the infected neurons, revealing the thin dendritic arborizations and the spines that are major post-synaptic seats of neuronal connections. Here, we comparatively illustrate the characteristics of the labeling obtained in the same model system, the basal ganglia circuitry, by different retrograde viral tracing approaches, using the Bartha strain of pseudorabies virus, the SAD and CVS strains of rabies virus and by the conventional retrograde tracer cholera toxin B. To best contrast the differences in the capacity of these tracers to reveal the dendritic morphology in details, we focused on one population of first-order infected neurons in the striatum, which exhibit high spine density, after tracer injection in the substantia nigra. None of the viruses tested allowed to detect as many neurons as with cholera toxin B, but the SAD and CVS strains of rabies virus ...Continue Reading

References

Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A M Graybiel, C W Ragsdale
Jul 1, 1990·Trends in Neurosciences·G E Alexander, M D Crutcher
Oct 1, 1989·Trends in Neurosciences·R L AlbinJ B Penney
Jan 1, 1997·The Journal of General Virology·H RauxD Blondel
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·K MorimotoB Dietzschold
Nov 14, 2000·Journal of Neuroscience Methods·G Aston-Jones, J P Card
Dec 21, 2006·Nature Methods·Ian R WickershamEdward M Callaway
May 23, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Stefano TavernaD James Surmeier
Apr 4, 2009·Neurobiology of Disease·Pascal SalinJosé L Lanciego
Apr 8, 2009·Current Opinion in Neurobiology·Edward M Callaway
Dec 17, 2009·Journal of Neuroscience Methods·Gabriella Ugolini
Jan 26, 2010·Journal of Neuroscience Methods·Iciar P LópezJosé L Lanciego
Nov 12, 2010·Journal of Virology·Christoph Wirblich, Matthias J Schnell
May 24, 2011·Advances in Virus Research·Gabriella Ugolini
Jun 12, 2012·Neuron·Mitsuko Watabe-UchidaNaoshige Uchida
Jul 6, 2014·Current Protocols in Neuroscience·J Patrick Card, Lynn W Enquist
Jul 30, 2014·Nature Neuroscience·Paolo CalabresiMassimiliano Di Filippo
Oct 16, 2014·Cerebral Cortex·Jaimie D AdelsonCarla J Shatz
Jun 19, 2015·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Edward M Callaway, Liqun Luo
Dec 4, 2015·Cold Spring Harbor Protocols·Nadin Hagendorf, Karl-Klaus Conzelmann

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