Gonadotropic control of secretion of dimeric inhibins and activin A by human granulosa-luteal cells in vitro

Journal of Assisted Reproduction and Genetics
S MuttukrishnaW Ledger

Abstract

It is well established that human granulosa cells and luteal cells express inhibin/activin subunit protein and secrete immunoreactive inhibin. The gonadotropic control of secretion of different molecular forms of inhibin and activin A by granulosa-luteal cells (G-LCs) was investigated using recently developed specific enzyme immunoassays (EIAs). Granulosa-luteal cells obtained at IVF egg pickup were cultured in a serum-free medium at 37 degrees C in a water-saturated incubator with 5% CO2 for up to 5 days. Experiments with varying concentrations of human FSH, hLH, and hCG were carried out. FSH raised the secretion of inhibin A and pro-alpha C-containing inhibins after 24 and 48 hr in culture. Inhibin B was raised after 24 hr and activin A was raised after 48 hr of FSH treatment. LH treatment for 24 hr stimulated inhibin A, inhibin B, pro-alpha C, and activin A. hCG stimulated G-LC secretion of inhibin A after 48 hr and pro-alpha C after 24 hr. Paradoxically, inhibin B secretion was inhibited by 1 and 10 ng/ml hCG after 48 hr. Activin A was stimulated by hCG after 24 and 48 hr of incubation. G-LC secretion of estradiol and progesterone was also stimulated significantly by LH and hCG. Secretion of dimeric inhibins and activin A i...Continue Reading

Citations

Jun 9, 2005·European Journal of Endocrinology·C BearfieldS Muttukrishna
May 8, 2008·Current Opinion in Obstetrics & Gynecology·Kevin S Richter
Sep 20, 2005·BJOG : an International Journal of Obstetrics and Gynaecology·S MuttukrishnaP Serhal
Oct 21, 2006·Journal of Assisted Reproduction and Genetics·Shiuh Young ChangKo-En Huang
May 28, 2011·Journal of Assisted Reproduction and Genetics·Katherine GelberYusuke Marikawa
Sep 29, 2004·Molecular and Cellular Endocrinology·Shanthi MuttukrishnaIan Sargent

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