Gonadotropin and steroid hormones regulate pluripotent very small embryonic-like stem cells in adult mouse uterine endometrium

Journal of Ovarian Research
Kreema JamesSiddhanath Metkari

Abstract

Very small embryonic-like stem cells (VSELs) exist in adult organs, express pluripotent markers and have the ability to differentiate into three germ layers in vitro. Testicular, ovarian and hematopoietic stem/progenitor cells express receptors for follicle stimulating (FSH) and ovarian hormones and are activated by them to undergo proliferation/differentiation. VSELs exist in mouse uterus and are regulated by physiological dose of estradiol (E) & progesterone (P) during endometrial growth, differentiation and regeneration/remodeling. In the present study, effects of daily administration of E (2 μg/day), P (1 mg/Kg/day) or FSH (5 IU/day) for 7 days on the endometrium and stem/progenitor cells was studied in bilaterally ovariectomized mice. E treatment resulted in hypertrophy whereas P resulted in hyperplasia and overcrowding of epithelial cells. FSH also directly stimulated the endometrial cells. Nuclear OCT-4A positive VSELs were visualized in ovariectomized (atrophied) endometrium and cytoplasmic OCT-4B positive epithelial, stromal and endothelial cells were observed after treatment. FSH treated uterine tissue showed presence of 4 alternately spliced FSHR isoforms by Western blotting. 3-5 μm VSELs with a surface phenotype of ...Continue Reading

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Citations

Nov 6, 2018·Endocrinology·Julie A W Stilley, Deborah L Segaloff
Aug 25, 2019·Stem Cell Research & Therapy·Hiren Patel, Deepa Bhartiya
Sep 25, 2019·Clinical Medicine Insights. Reproductive Health·Zeev Blumenfeld
Jan 23, 2021·Stem Cell Research & Therapy·Deepa Bhartiya
Nov 7, 2020·Stem Cell Reviews and Reports·Malak El SabehMostafa Borahay

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Methods Mentioned

BETA
flow cytometry
PCR
FACS
Flow

Software Mentioned

FACS Diva
CFX Manager

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