Gonadotropin regulation of activin betaA and activin type IIA receptor expression in the ovarian follicle cells of the zebrafish, Danio rerio

Molecular and Cellular Endocrinology
Yefei Pang, Wei Ge

Abstract

We have previously demonstrated that both activin and its receptors are expressed in the zebrafish ovary, suggesting paracrine roles for activin in the ovarian functions. Activin significantly stimulated zebrafish oocyte maturation in vitro, and this effect could be blocked by follistatin, an activin-binding protein. Interestingly, follistatin also blocked the stimulatory effect of gonadotropin (hCG) on the oocyte maturation. Taken together, these results have led to a hypothesis that the ovarian activin system may play a role in mediating the actions of gonadotropin in the ovary. To test this hypothesis, the present study was undertaken to investigate if gonadotropin has any effect on the expression of activin betaA subunit and activin type IIA (ActRIIA) receptor in the zebrafish ovary. A primary culture of zebrafish ovarian follicle cells was established in the present study, and the cultured cells expressed both activin betaA and ActRIIA receptor when assayed with RT-PCR. The primary culture consisted of three major types of cells, presumably the fibroblasts, the thecal cells and the granulosa cells, according to the morphological features, histochemical staining for 3beta-hydroxysteroid dehydrogenase (3beta-HSD) and RT-PCR ...Continue Reading

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Citations

Nov 18, 2011·Biology of Reproduction·Giorgia GioacchiniOliana Carnevali
Jul 16, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Shui-Kei PoonWei Ge
Dec 15, 2015·General and Comparative Endocrinology·Rui ZhouWei Ge
Oct 8, 2008·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Qian TanChun Peng
Oct 27, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·Debabrata DasSudipta Maitra
Sep 1, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·Maria L SousaRalph Urbatzka
Oct 29, 2008·General and Comparative Endocrinology·Ana Lupe Motta StudzinskiLuis Fernando Marins
Nov 19, 2010·Biology of Reproduction·Jianzhen LiChristopher H K Cheng
Mar 7, 2017·Scientific Reports·Christopher PresslauerIgor Babiak
May 2, 2009·Molecular and Cellular Endocrinology·Eric Clelland, Chun Peng
Sep 1, 2007·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Márcio de Azevedo FigueiredoLuis Fernando Marins
Mar 4, 2017·Molecular and Cellular Endocrinology·Haipei TangHaoran Lin
May 16, 2006·Molecular Reproduction and Development·Jennifer S Ings, Glen J Van Der Kraak
Apr 1, 2005·Fish Physiology and Biochemistry·Wei Ge
Jun 19, 2012·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Juhua ChangGuonian Zhu
Jun 27, 2018·Biology of Reproduction·Jianzhen LiChristopher H K Cheng
Apr 10, 2012·General and Comparative Endocrinology·David A Irwin, Glen Van Der Kraak
Jun 10, 2009·General and Comparative Endocrinology·Esther LubzensJoan Cerdà

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