Gonadotropin release and redistribution of calcium-activated, phospholipid-dependent protein kinase in phorbol-stimulated rat pituitary cells

Archives of Biochemistry and Biophysics
K HirotaK J Catt

Abstract

The effect of phorbol esters on calcium-activated, phospholipid-dependent kinase (protein kinase C) and luteinizing hormone (LH) secretion was examined in cultured rat anterior pituitary cells. The potent tumor promoter 12-O-tetra-decanoylphorbol-13-acetate (TPA) stimulated LH secretion and activated pituitary protein kinase C in the presence of calcium and phosphatidylserine. The enzyme activity present in cytosol and particulate fractions was eluted at about 0.05 M NaCl during DE52-cellulose chromatography. Preincubation of pituitary cells with TPA markedly decreased cytosolic protein kinase C activity and increased enzyme activity in the particulate fraction. The maximal TPA-induced change in enzyme activity, with a 76% decrease in cytosol and a 4.3-fold increase in the particulate fraction, occurred within 10 min. The dose-dependent changes in protein kinase C redistribution in TPA-treated cells were correlated with the stimulation of LH release by the phorbol ester. These results suggest that activation of protein kinase C by TPA is associated with intracellular redistribution of the enzyme and is related to the process of secretory granule release from gonadotrophs.

References

Feb 4, 1979·Biochimica Et Biophysica Acta·A Sivak
Jan 1, 1974·CRC Critical Reviews in Toxicology·R K Boutwell
Jul 18, 1984·Biochemical and Biophysical Research Communications·P M Tapley, A W Murray
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J E NiedelG R Vandenbark
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·K L LeachP M Blumberg
Jan 1, 1983·Annals of the New York Academy of Sciences·P M BlumbergE Yeh
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S JacobsP Cuatrecasas
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·N A SharkeyP M Blumberg
May 15, 1984·The Biochemical Journal·K W Brocklehurst, J C Hutton
Apr 29, 1983·Biochemical and Biophysical Research Communications·J YamanishiY Nishizuka
Feb 28, 1983·Biochemical and Biophysical Research Communications·C L AshendelR K Boutwell
Oct 1, 1983·The Journal of Clinical Endocrinology and Metabolism·M R ClarkW J Lemaire
Apr 1, 1980·Cell·K B DelclosP M Blumberg

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Citations

Aug 1, 1991·Molecular and Cellular Endocrinology·M B FrenchJ Kraicer
Apr 21, 2010·Fertility and Sterility·Daniel J BernardPankaj Lamba

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