Gonadotropin-releasing hormone-independent effects of recombinant vertebrate ancient long opsin in the goldfish Carassius auratus reveal alternative reproduction pathways.

Fish Physiology and Biochemistry
Ji Yong Choi, Cheol Young Choi

Abstract

Vertebrate ancient long (VAL)-opsin is a green-sensitive photoreceptor that shows high sequence similarity to vertebrate ancient opsin, which is considered to play a role in sexual maturation via gonadotropin-releasing hormone (GnRH); however, the role of VAL-opsin in vertebrate sexual maturity remains unclear. Therefore, we investigated the possible role of VAL-opsin in reproduction in the goldfish Carassius auratus under a state of GnRH inhibition. Goldfish were injected with recombinant VAL-opsin protein (0.5 μg/g body mass) and/or the GnRH antagonist cetrorelix (0.5 μg/fish), and changes in the mRNA expression levels of genes associated with goldfish reproduction were measured by quantitative polymerase chain reaction, including those involved in the hypothalamus-pituitary-gonad (HPG) axis, VAL-opsin, GnRH, the gonadotropins (GTHs) luteinizing hormone and follicle-stimulating hormone, and estrogen receptor (ER). Moreover, the fish were irradiated with a green light-emitting diode (520 nm) to observe the synergistic effect on the HPG axis with VAL-opsin. Green LED exposure significantly and slightly increased the VAL-opsin and GnRH levels, respectively; however, these effects were blocked in groups injected with cetrorelix a...Continue Reading

References

Jan 1, 1995·Current Topics in Developmental Biology·Y NagahamaY Katsu
Apr 14, 1997·FEBS Letters·B G Soni, R G Foster
Sep 6, 2000·Human Reproduction Update·T ReissmannJ Engel
Jun 12, 2001·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Y H LeeC F Chang
Jan 10, 2002·Biochemical and Biophysical Research Communications·Toshifumi Minamoto, Isamu Shimizu
Oct 19, 2002·Molecular and Cellular Endocrinology·Christopher J BowmanNancy D Denslow
Nov 27, 2007·Journal of Neurochemistry·Daisuke KojimaJohn E Dowling
Aug 12, 2009·Current Biology : CB·Stephanie HalfordRussell G Foster
Aug 4, 2010·Proceedings of the National Academy of Sciences of the United States of America·Yusuke NakaneTakashi Yoshimura
Jan 6, 2011·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Rita BerardelliEmanuela Arvat
May 5, 2011·Chronobiology International·B Blanco-VivesF J Sánchez-Vázquez
Oct 28, 2011·Biology Letters·Wayne I L DaviesRussell G Foster
May 9, 2012·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Na Na KimCheol Young Choi
Dec 26, 2013·Zebrafish·Natalia VillamizarFrancisco Javier Sánchez-Vázquez
Dec 3, 2014·Frontiers in Neuroendocrinology·José M García-FernándezRussell G Foster
Jun 4, 2016·Biochemical and Biophysical Research Communications·Jin Ah SongCheol Young Choi
Apr 7, 2017·Fish Physiology and Biochemistry· Vijayalaxmi, C B Ganesh

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Citations

Oct 13, 2020·Frontiers in Neuroendocrinology·Hsien-Ming WuPeter C K Leung

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Methods Mentioned

BETA
PCR

Software Mentioned

Beacon Designer
BLAST

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