Gonadotropin-releasing hormone-induced stimulation and desensitization of free alpha-subunit secretion mirrors luteinizing hormone and follicle-stimulating hormone in perifused rat pituitary cells

Endocrinology
J WeissW F Crowley

Abstract

A pulsatile pattern of hypothalamic GnRH stimulation is necessary for the maintenance of pituitary LH and FSH secretion, with continuous GnRH leading to a decrement in response. Although the physiological pattern of free alpha-subunit secretion closely mimics that of LH, several reports have indicated that free alpha-subunit is not desensitized by continuous GnRH stimulation. To explore the basis of this phenomenon, we have evaluated the responses of all three gonadotrope secretory products to carefully coordinated administration of pulsatile and continuous GnRH in a dispersed rat pituitary perifusion system. Sensitivities (ED50) to GnRH fell within a narrow range for free alpha-subunit (11.5 nM), LH (12.9 nM), and FSH (17.3 nM), although a greater mass of LH than free alpha-subunit or FSH was released after each pulse of GnRH. The response to a standard GnRH pulse (10 nM) administered every 15, 30, or 120 min for 9 h was very stable, with no evidence of priming, summation, or loss of response. LH, FSH, and free alpha-subunit did, however, show significantly (P less than 0.05) higher pulse amplitude with longer interpulse intervals. In contrast to previous observations in vivo, the three gonadotrope secretory products showed pa...Continue Reading

Citations

Jun 1, 1994·The Journal of Clinical Investigation·J M Alexander, A Klibanski
Sep 24, 2013·Molecular and Cellular Endocrinology·Iain R Thompson, Ursula B Kaiser
Aug 19, 2008·Frontiers in Neuroendocrinology·Zvi Naor
Oct 1, 1993·Trends in Endocrinology and Metabolism : TEM·J Weiss, J Larry Jameson
Jul 1, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S TanakaK Wakabayashi
May 28, 1993·Annals of the New York Academy of Sciences·J E HallW F Crowley
Feb 3, 2016·Molecular Endocrinology·Iain R ThompsonUrsula B Kaiser
Dec 14, 2006·The Journal of Clinical Endocrinology and Metabolism·Yardena Tenenbaum-RakoverNicolas de Roux
Apr 3, 2014·The Journal of Biological Chemistry·Michael P BernardWilliam R Moyle

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