Gonadotropin-releasing hormone regulates follicle-stimulating hormone beta-subunit gene expression in the male rat

Endocrinology
D A RodinR N Clayton

Abstract

Since the role of GnRH in the control of FSH release and synthesis is controversial, we have examined the effect of elimination of GnRH action on gonadotropes on FSH beta gene expression, FSH release, and synthesis. GnRH stimulation of the pituitary was abolished by continuous infusion of either a GnRH antagonist or a GnRH antiserum. We also examined the effects of gonadotrope desensitization, using a continuous infusion of GnRH or GnRH agonist analog. FSH beta mRNA levels were determined by dot blot hybridization using rat FSH beta cDNA, and changes were related to pituitary and serum FSH concentrations. FSH beta mRNA levels increased after orchidectomy and correlated well with serum FSH concentrations. Overall FSH synthesis was increased after castration, as judged by elevated serum FSH and unchanged pituitary FSH content. In orchidectomized rats, continuous GnRH antagonist infusion prevented the postcastration rise in FSH beta mRNA levels and serum FSH. Pituitary FSH content was reduced at 7 days, but not at 14 days. In intact rats, GnRH antagonist infusion for 7 days had no effect on FSH beta mRNA levels, but after 14 days, there was a 33% reduction, and serum FSH was suppressed. Pituitary FSH content was decreased after Gn...Continue Reading

Citations

Jul 1, 1995·Trends in Endocrinology and Metabolism : TEM·M Bergendahl, J D Veldhuis
Jul 3, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·Stephen J Winters, Joseph P Moore
Jul 1, 1992·American Journal of Obstetrics and Gynecology·T UemuraH Minaguchi
Jun 16, 1999·Endocrine Reviews·J F Couse, K S Korach
Apr 19, 2005·Treatments in Endocrinology·Juergen M WeissMichael Ludwig
May 1, 1993·The American Journal of Physiology·M Bergendahl, I Huhtaniemi

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