Gonadotropin-releasing hormone type II (GnRH-II) agonist regulates the motility of human decidual endometrial stromal cells: possible effect on embryo implantation and pregnancy

Biology of Reproduction
Hsien-Ming WuHsin-Shih Wang

Abstract

Invasion of the maternal decidua by extravillous trophoblast is an important process for embryo implantation and placentation in humans. Motile behavior of decidual endometrial stromal cells has been considered of critical importance for embryo implantation and programming of human pregnancy. The gonadotropin-releasing hormone (GnRH) effects in endometrium have raised concerns in reproduction. In the present study, we examined the action of GnRH-II agonist-promoted motility of human decidual endometrial stromal cells and the mechanisms of the action, indicating the role of GnRH-II agonist in embryo implantation and early pregnancy. Human decidual endometrial stromal cells were isolated from the decidual tissue from healthy women undergoing elective pregnancy termination of a normal pregnancy at 6- to 12-wk gestation, after informed consent. Cell motility was estimated by invasion and migration assay. Zymography and immunoblot analysis were performed to investigate the mechanisms of the GnRH-II action. The GnRH-I receptor (GnRH-IR) was expressed in human decidual tissue and endometrial stromal cells. The GnRH-II agonist promoted cell motility. Mitogen-activated protein kinase inhibitors abolished GnRH-II agonist-induced cell mot...Continue Reading

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Citations

Oct 17, 2019·Animals : an Open Access Journal From MDPI·Yongfu LaMingxing Chu
Oct 13, 2020·Frontiers in Neuroendocrinology·Hsien-Ming WuPeter C K Leung
Dec 18, 2020·Frontiers in Immunology·Gabriel Mayoral AndradeEduardo Pérez-Campos
Jul 17, 2019·Current Medicinal Chemistry·Haralambos TzoupisTheodore Tselios
Jul 4, 2021·Tissue & Cell·Carlo TicconiAsgerally Fazleabas

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Methods Mentioned

BETA
mechanical dissociation
protein assay
electrophoresis
PCR
transfection
zymography

Software Mentioned

iQ5 Optical System

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