Gonadotropins, serum, and amino acids alter nuclear maturation, cumulus expansion, and oocyte morphology in hamster cumulus-oocyte complexes in vitro

Biology of Reproduction
S Kito, B D Bavister

Abstract

Glucose, lactate, and pyruvate (the substrate triad), gonadotropins, serum, and amino acids were tested on maturation of cumulus-oocyte complexes (COCs) using a simple defined medium, Tyrode's-PVA (T-PVA). In experiment 1, effects of FSH (2 microg/ml) and the substrate triad were tested using a 2 x 2 factorial design. After 12-13 h, nuclear maturation was depressed in the absence of the triad or with FSH (0-14% metaphase II [MII]) compared with the triad alone (92% MII, p < 0.05). Subsequent experiments used as the base medium Tyrode's solution with the triad (TLP-PVA): adding 10% bovine calf serum (BCS) and gonadotropins (10 microg/ml FSH, 10 microg/ml LH, or both) yielded nuclear maturation equivalent to that in medium alone (88-100% post-metaphase I [post-MI] oocytes). Responses with glutamine, or with 11 but not 20 amino acids, were equivalent to the response in BCS with gonadotropins (93-100% post-MI oocytes). Some cumulus expansion occurred in COCs matured with gonadotropins and BCS, or glutamine, or 11 amino acids, but was less extensive than for in vivo-matured COCs. Oocytes matured with gonadotropins and BCS, or glutamine, or 11 amino acids plus gonadotropins, but not medium alone, had normal-appearing first polar bodi...Continue Reading

Citations

May 17, 2000·Molecular Reproduction and Development·R E SpindlerD E Wildt
Mar 3, 2004·Reproductive Biomedicine Online·Ri-Cheng ChianSeang-Lin Tan
Jun 27, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Xiaoqian WangPeter Temple-Smith
Jul 15, 2015·Zygote : the Biology of Gametes and Early Embryos·Letícia Ferrari CrocomoSony Dimas Bicudo
Oct 5, 2014·Cellular and Molecular Life Sciences : CMLS·Ling GuQiang Wang
Apr 4, 2006·Theriogenology·B A Rodrigues, J L Rodrigues
Aug 4, 2009·Trends in Endocrinology and Metabolism : TEM·Lacey J LuenseLane K Christenson
Jun 13, 2015·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Jiancao GaoZaizhao Wang
Mar 30, 2007·Zygote : the Biology of Gametes and Early Embryos·B de Avila RodriguesJ L Rodrigues

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