Gossypol repressed the gap junctional intercellular communication between Sertoli cells by decreasing the expression of Connexin43

Toxicology in Vitro : an International Journal Published in Association with BIBRA
De-Rong ZhouZhi-Ming Cai

Abstract

Previous studies showed that gossypol could block the gap junctional intercellular communication (GJIC) between cultured cells. The present study was designed to investigate the effects of gossypol on the GJIC and the expression of connexin43 (Cx43) in the cultured cells. A Sertoli cell line, TM4, was treated with different concentrations of gossypol 1.25, 2.5, 5, and 10micromol/L for 6, 12, 24, and 48h. Cell viability was assessed with CCK-8 assay. GJIC in the cells was determined using the scrape loading and dye transfer (SLDT) assay; the expression of Cx43 was detected by RT-PCR, immunofluorescence and Western blot analysis. The SLDT assay showed gossypol significantly decreased GJIC between adjacent cells. RT-PCR, immunofluorescence and Western blot analyses demonstrated the expression of Cx43 in TM4 cells. The expression of Cx43 was gradually decreased with the increasing concentrations of gossypol, and the effect occurred as early as 6h after the treatment and continued until 48h. These results suggested that gossypol impaired GJIC by decrease of Cx43 expression in the cells, which is important for Sertoli cells to regulate spermatogenesis.

References

Jan 1, 1979·Tissue & Cell·D M McGinleyL Russell
Sep 12, 1992·BMJ : British Medical Journal·E CarlsenN E Skakkebaek
Oct 1, 1989·International Journal of Peptide and Protein Research·T Strøm-HansenJ W Jaroszewski
Jun 1, 1986·Clinical Pharmacology and Therapeutics·D F WuM Z Wang
Jan 1, 1984·Annual Review of Pharmacology and Toxicology·S Z Qian, Z G Wang
Sep 26, 1995·Proceedings of the National Academy of Sciences of the United States of America·G Sosinsky
Mar 1, 1993·The Journal of Endocrinology·R M Sharpe
May 15, 1996·European Journal of Biochemistry·R BruzzoneD L Paul
Oct 17, 1996·European Journal of Pharmacology·J C HervéJ Délèze
Aug 25, 1999·Molecular Human Reproduction·Y GuiB A Lessey
Apr 20, 2000·Biochimica Et Biophysica Acta·J M CristanchoW A Varanda
May 23, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C BatiasD Segretain
Sep 21, 2000·Current Biology : CB·A PlumK Willecke
Oct 6, 2000·Andrologia·T K MonseesJ Hayatpour
Aug 22, 2001·Biology of Reproduction·W A RoscoeG M Kidder
Mar 22, 2003·Laboratory Investigation; a Journal of Technical Methods and Pathology·Norah DefamieGeorges Pointis
Apr 15, 2004·Reproductive Toxicology·Céline FioriniGeorges Pointis
Jun 27, 2007·The American Journal of Pathology·Ralph BrehmMartin Bergmann

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