Got black swimming dots in your cell culture? Identification of Achromobacter as a novel cell culture contaminant.

Biologicals : Journal of the International Association of Biological Standardization
Jennifer Sue GrayJenifer Imig Fenton

Abstract

Cell culture model systems are utilized for their ease of use, relative inexpensiveness, and potentially limitless sample size. Reliable results cannot be obtained, however, when cultures contain contamination. This report discusses the observation and identification of mobile black specks observed in multiple cell lines. Cultures of the contamination were grown, and DNA was purified from isolated colonies. The 16S rDNA gene was PCR amplified using primers that will amplify the gene from many genera, and then sequenced. Sequencing results matched the members of the genus Achromobacter, bacteria common in the environment. Achromobacter species have been shown to be resistant to multiple antibiotics. Attempts to decontaminate the eukaryotic cell culture used multiple antibiotics at different concentrations. The contaminating Achromobacter was eventually eliminated, without permanently harming the eukaryotic cells, using a combination of the antibiotics ciprofloxacin and piperacillin.

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Citations

Jan 7, 2015·Journal of Cellular Biochemistry·Muhammad Tahir AsgharAbdul Rauf Shakoori
Sep 11, 2018·Acta Crystallographica. Section F, Structural Biology Communications·Abhisek DwivedyBichitra Kumar Biswal
Feb 2, 2018·Scientific Reports·Ji-Hyun YunJin-Woo Bae
Sep 8, 2019·Environmental Science and Pollution Research International·Haichao WangChuanyong Jing
Mar 22, 2013·The Journal of Biological Chemistry·Soroush TahmasebiXiang-Jiao Yang
Jul 25, 2019·World Journal of Microbiology & Biotechnology·Sarah ZecchinLucia Cavalca
Mar 20, 2019·Molecular Ecology Resources·Laura S WeyrichAlan Cooper
Jun 6, 2018·Cell Medicine·John E PiletzKellie Brue
May 1, 2020·The Journal of Investigative Dermatology·Alexander SalavaAntti Lauerma

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