Got Milk? Identifying and Characterizing Lactation Defects in Genetically-Engineered Mouse Models.

Journal of Mammary Gland Biology and Neoplasia
Teneale A Stewart, Felicity M Davis

Abstract

The ability to produce and expel milk is important for the health and survival of all mammals. Nevertheless, our understanding of the molecular events underlying the execution of this process remains incomplete. Whilst impaired mammary gland development and lactational competence remains the subject of focused investigations, defects in these events may also be an unintended consequence of genetic manipulation in rodent models. In this technical report, we outline established and emerging methods to characterize lactation phenotypes in genetically-engineered mouse models. We discuss important considerations of common models, optimized conditions for mating and the importance of litter size and standardization. Methods for quantifying milk production and quality, as well as protocols for wholemount preparation, immunohistochemistry and the preparation of RNA and protein lysates are provided. This review is intended to help guide researchers new to the field of mammary gland biology in the systematic analysis of lactation defects and in the preparation of samples for more focused mechanistic investigations.

References

Sep 8, 1995·Science·R KühnK Rajewsky
Oct 23, 1997·Nucleic Acids Research·K U WagnerL Hennighausen
Dec 22, 1998·Transgenic Research·S SelbertA R Clarke
Aug 11, 2004·The Journal of Biological Chemistry·Timothy A ReinhardtRonald L Horst
Dec 1, 2006·Journal of Mammary Gland Biology and Neoplasia·Carol A PalmerJames L McManaman
Dec 28, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Eon Joo ParkAnne M Moon
May 1, 2009·Journal of Mammary Gland Biology and Neoplasia·Derek C Radisky, Lynn C Hartmann
Nov 26, 2009·Journal of Mammary Gland Biology and Neoplasia·Edward J DePeters, Russell C Hovey
Nov 26, 2009·Journal of Mammary Gland Biology and Neoplasia·John D LanduaMichael T Lewis
Mar 6, 2010·Methods in Molecular Biology·Matthew J Smalley
May 21, 2010·Cold Spring Harbor Perspectives in Biology·Pamela Cowin, John Wysolmerski
Sep 14, 2010·Nature Methods·Jane L Hurst, Rebecca S West
Dec 14, 2011·The International Journal of Developmental Biology·Christine J Watson, Peter A Kreuzaler
May 31, 2012·Journal of Mammary Gland Biology and Neoplasia·Matthew J SmalleyJohn Stingl
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Jul 24, 2012·Current Protocols in Mouse Biology·Tanya BurkholderJoanne M Smith
Jul 28, 2012·The Journal of Experimental Biology·Shelley L C LangW Don Bowen
Jan 9, 2014·Breast Cancer Research : BCR·Ted H-T ChangAlexandra M Pietersen
Jan 28, 2014·Nature·Anne C RiosJane E Visvader
Apr 23, 2015·Proceedings of the National Academy of Sciences of the United States of America·Felicity M DavisJames W Putney
May 12, 2015·Cell Reports·Camila O Dos SantosGregory J Hannon
Aug 3, 2016·Cold Spring Harbor Protocols·Richard BehringerAndras Nagy
Dec 15, 2016·Breast Cancer Research : BCR·Bethan Lloyd-LewisChristine J Watson
Dec 15, 2016·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Felicity M Davis
Jan 31, 2017·Nature·Colinda L G J ScheeleJacco van Rheenen
Feb 9, 2017·Journal of Mammary Gland Biology and Neoplasia·Ingrid S Paine, Michael T Lewis
May 11, 2017·Trends in Cell Biology·Bethan Lloyd-LewisFelicity M Davis
May 12, 2018·Nature Protocols·Andrew J ModzelewskiLin He
May 23, 2018·Nature Cell Biology·Aline WuidartCédric Blanpain
Jul 27, 2018·Development·Bethan Lloyd-LewisChristine J Watson
Feb 23, 2019·Methods in Molecular Biology·Julie M Sheridan, Jane E Visvader
Jun 30, 2019·Laboratory Animals·Nikita VedDuncan Burnaby Sparrow

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Citations

Jan 23, 2021·Journal of Mammary Gland Biology and Neoplasia·Teresa MonkkonenZuzana Koledova
Apr 25, 2021·Seminars in Cell & Developmental Biology·Felicity M Davis

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