gp180, a protein that binds duck hepatitis B virus particles, has metallocarboxypeptidase D-like enzymatic activity.

The Journal of Biological Chemistry
F J EngL D Fricker

Abstract

Duck gp180 was previously identified by its ability to bind to the preS envelope protein of duck hepatitis B virus particles (Kuroki, K. , Cheung, R., Marion, P. L., and Ganem, D. (1994) J. Virol. 68, 2091-2096). Cloning and sequencing of gp180 cDNA revealed that it is a polyprotein with three carboxypeptidase-like domains (Kuroki, K., Eng, F., Ishikawa, T., Turck, C., Harada, F., and Ganem, D. (1995) J. Biol. Chem. 270, 15022-15028). To evaluate enzymatic properties of this protein, a soluble 170-kDa form of the protein (gp170) lacking the C-terminal transmembrane domain and cytoplasmic tail was expressed in a baculovirus system. The purified 170-kDa protein cleaved 5-dimethylaminonaphthalene-1-sulfonyl (dansyl)-Phe-Ala-Arg with a pH optimum of 5.5-6.5. With this substrate at pH 5.5, the 170-kDa protein displayed a Km of 12 microM and a Kcat of 57 s-1. Dansyl-Pro-Ala-Arg and dansyl-Phe-Phe-Arg were cleaved with Km values of 17 and 21 microM, and Kcat values of 57 and 17 s-1, respectively. Constructs containing only the first or second carboxypeptidase domains also showed enzymatic activity. The effects of inhibitors and ions on enzyme activity of gp170 were generally similar to the effects of these compounds on purified bovine...Continue Reading

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Citations

Apr 14, 2010·Cellular and Molecular Life Sciences : CMLS·Galyna SidyelyevaLloyd D Fricker
Jul 21, 2007·Journal of Clinical Immunology·Norman-Philipp HoffJens Ulrich Wurthner
Sep 29, 1999·Trends in Biochemical Sciences·L D Fricker, E H Leiter
Dec 20, 2002·International Immunopharmacology·Barbara TimblinRandal A Skidgel
Jul 16, 2004·Journal of Virology·Kathie-Anne WaltersD Lorne J Tyrrell
Jul 15, 2005·Journal of Virology·Azeneth BarreraRobert E Lanford
Jul 23, 2013·Virology Journal·Wolfram H Gerlich
Apr 21, 2016·ACS Medicinal Chemistry Letters·Uno TagamiToshimi Mizukoshi
Nov 19, 2008·Journal of Neurochemistry·Xin ZhangLloyd D Fricker
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Oct 11, 2013·The Journal of Biological Chemistry·Xianming ZhangRandal A Skidgel
Feb 12, 2002·The Journal of Biological Chemistry·Suwen WeiLloyd D Fricker
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Nov 3, 2015·Clinical and Molecular Hepatology·Jisu Li, Shuping Tong
Oct 24, 2002·The Journal of Biological Chemistry·Galyna Sidyelyeva, Lloyd D Fricker
Mar 30, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S SpijkerW P Geraerts
Oct 9, 2014·The Journal of Biological Chemistry·Javier Garcia-PardoSalvador Ventura

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