GPHR-dependent functions of the Golgi apparatus are essential for the formation of lamellar granules and the skin barrier

The Journal of Investigative Dermatology
Masahito TarutaniYusuke Maeda

Abstract

The lumen of the Golgi apparatus is regulated to be weakly acidic, which is critical for its functions. The Golgi pH regulator (GPHR) is an anion channel essential for normal acidification of the Golgi apparatus, and is therefore required for its functions. The Golgi apparatus has been thought to be the origin of lamellar granules in the skin. To study the functional role(s) of GPHR in the skin, we established keratinocyte-specific GPHR-knockout mice using the Cre-loxP system. These mutant mice exhibited hypopigmented skin, hair loss, and scaliness. Histological examination of GPHR-knockout mice showed ballooning of the basal cells and follicular dysplasia. In addition, inflammatory cells were seen in the dermis. The expression of trans-Golgi network 46, a marker for lamellar bodies, and kallikrein 7, a protein within lamellar bodies, is diminished in GPHR-knockout mouse skin. Examination by electron microscopy revealed that keratinocytes produced aberrant lamellar bodies. The transepidermal water loss of these knockout mice was increased compared with wild-type mice. Moreover, expression of cathelicidin-related antimicrobial peptide (CRAMP) in the skin was diminished. These results suggest that GPHR is essential for the homeos...Continue Reading

References

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Citations

Jul 17, 2012·The Journal of Investigative Dermatology·Kenneth R Feingold
Feb 14, 2016·The Journal of Investigative Dermatology·Marie ReynierCorinne Leprince
Jul 28, 2016·Cellular and Molecular Life Sciences : CMLS·Carmen Ehrmann, Marlon R Schneider
May 27, 2017·Disease Models & Mechanisms·Maria StumpfAngelika A Noegel
May 9, 2013·Experimental Dermatology·Junichi KumamotoMitsuhiro Denda
Aug 31, 2021·Frontiers in Cell and Developmental Biology·Sarmistha Mahanty, Subba Rao Gangi Setty

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