GPI anchor biosynthesis in yeast: phosphoethanolamine is attached to the alpha1,4-linked mannose of the complete precursor glycophospholipid

Glycobiology
E Canivenc-GanselA Benachour

Abstract

Cells synthesize the GPI anchor carbohydrate core by successively adding N-acetylglucosamine, three mannoses, and phosphoethanolamine (EtN-P) onto phosphatidylinositol, thus forming the complete GPI precursor lipid which is then added to proteins. Previously, we isolated a GPI deficient yeast mutant accumulating a GPI intermediate containing only two mannoses, suggesting that it has difficulty in adding the third, alpha1,2-linked Man of GPI anchors. The mutant thus displays a similar phenotype as the mammalian mutant cell line S1A-b having a mutation in the PIG-B gene. The yeast mutant, herein named gpi10-1 , contains a mutation in YGL142C, a yeast homolog of the human PIG-B. YGL142C predicts a highly hydrophobic integral membrane protein which by sequence is related to ALG9, a yeast gene required for adding Man in alpha1,2 linkage to N-glycans. Whereas gpi10-1 cells grow at a normal rate and make normal amounts of GPI proteins, the microsomes of gpi10-1 are completely unable to add the third Man in an in vitro assay. Further analysis of the GPI intermediate accumulating in gpi10 shows it to have the structure Manalpha1-6(EtN-P-)Manalpha1-4GlcNalpha1-6(acyl) Inositol-P-lipid. The presence of EtN-P on the alpha1,4-linked Man of ...Continue Reading

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Citations

Dec 5, 2000·Current Opinion in Chemical Biology·T Kinoshita, N Inoue
Apr 10, 2010·Glycobiology·Françoise Debierre-Grockiego, Ralph T Schwarz
Aug 30, 2002·Molecular Biology and Evolution·Rafael OriolPatrice Codogno
Jun 29, 1999·Biological Chemistry·A TiedeR E Schmidt
Nov 9, 2001·Biochemical and Biophysical Research Communications·D SevleverM E Medof
Jan 28, 2015·FEBS Letters·Yohei OhnumaAkira Kakizuka
Jul 28, 2011·The FEBS Journal·Eleonora AltmanEvgeny Vinogradov
Feb 10, 2010·Chembiochem : a European Journal of Chemical Biology·Yug Varma, Tamara Hendrickson
Jul 25, 2006·Biochimica Et Biophysica Acta·Martine Pittet, Andreas Conzelmann
Nov 26, 1999·Biochimica Et Biophysica Acta·M A FergusonT K Smith
Apr 3, 2014·Molecular & Cellular Proteomics : MCP·Weixuan ChenRonghu Wu
Apr 26, 2003·The Journal of Biological Chemistry·Mariko UmemuraYoshifumi Jigami
Jul 19, 2003·The Journal of Biological Chemistry·Isabelle GuillasAndreas Conzelmann
May 29, 2003·Biochimie·Cristiana Santos de MacedoNahid Azzouz

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