GPI (glycosylphosphatidylinositol)-linked aspartyl proteases regulate vacuole homoeostasis in Candida glabrata

The Biochemical Journal
Gaurav BairwaRupinder Kaur

Abstract

A family of 11 GPI (glycosylphosphatidylinositol)-linked cell surface-associated aspartyl proteases (yapsins) in the human opportunistic fungal pathogen Candida glabrata is required for cell wall remodelling, pH homoeostasis, survival in macrophages and virulence in a murine model of disseminated candidiasis. In the present paper, we report new roles for yapsins in C. glabrata physiology and implicate them for the first time in the regulation of vacuole homoeostasis. In the present study we show that a C. glabrata mutant lacking all 11 yapsins, Cgyps1-11∆, possesses an enlarged vacuole and displays vma- (vacuolar membrane ATPase)-like phenotypes with elevated metal ion susceptibility in an alkaline pH medium and diminished Vma activity. The results of the present study also demonstrate a singular role for CgYps1 (C. glabrata yapsin 1) in the maintenance of ion homoeostasis under normal and calcineurin-inhibited conditions. Elevated polyphosphate levels and diminished cellular CPY (carboxypeptidase Y) activity in the Cgyps1-11∆ mutant highlight the yapsin requirement for a properly functioning vacuole. Lastly, a gross perturbation of cellular homoeostasis in the Cgyps1-11∆ mutant, even in the absence of external stressors, chara...Continue Reading

References

Sep 1, 1990·Microbiological Reviews·D J KlionskyS D Emr
Oct 3, 2001·Molecular Microbiology·O BaderB Hube
Dec 12, 2001·Molecular Microbiology·T EricksonP R Williamson
Jun 21, 2002·Methods in Enzymology·Elizabeth W Jones
Apr 16, 2003·Genome Research·Irene CastanoBrendan P Cormack
May 16, 2003·Molecular and Cellular Biology·Daniel W Neef, Michael P Kladde
Jul 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·Sukkid YasothornsrikulVivian Hook
Aug 23, 2003·Infection and Immunity·Teresa BaderJoachim Morschhäuser
Sep 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Julian R NaglikBernhard Hube
Jul 2, 2004·Nature·Bernard DujonJean-Luc Souciet
Aug 13, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Hilmar WisplinghoffMichael B Edmond
Feb 8, 2005·Current Opinion in Microbiology·Jorge E Galán, Pascale Cossart
Jul 6, 2005·Current Opinion in Microbiology·Rupinder KaurBrendan P Cormack
Jan 21, 2006·The Journal of Biological Chemistry·Sergio Padilla-López, David A Pearce
Oct 18, 2006·FEMS Yeast Research·Isabelle Gagnon-ArsenaultYves Bourbonnais
Apr 26, 2007·Proceedings of the National Academy of Sciences of the United States of America·Rupinder KaurBrendan P Cormack
Nov 15, 2007·Journal of Bioenergetics and Biomembranes·Patricia M Kane
Sep 13, 2008·Biochimica Et Biophysica Acta·Sheena Claire Li, Patricia M Kane
Feb 3, 2009·Methods in Enzymology·Margarita Cabrera, Christian Ungermann
Apr 7, 2009·Annual Review of Biochemistry·Narayana N RaoArthur Kornberg
Jun 24, 2009·Memórias do Instituto Oswaldo Cruz·B Parra-OrtegaC Hernández-Rodríguez
Jan 27, 2010·Antimicrobial Agents and Chemotherapy·Taiga MiyazakiShigeru Kohno
May 11, 2010·Biochemistry·Masashi ToeiMichael Forgac
Dec 15, 2010·Applied Microbiology and Biotechnology·Hiroyuki MukaiyamaKaoru Takegawa
Aug 13, 2011·The Journal of Biological Chemistry·Sapan BorahRupinder Kaur
Jan 4, 2012·The American Journal of Medicine·Michael A Pfaller
Jun 13, 2012·G3 : Genes - Genomes - Genetics·Ying-Lien ChenJoseph Heitman

❮ Previous
Next ❯

Citations

Oct 1, 2014·Mycoses·Ewa Swoboda-KopećIrina Netsvyetayeva
Sep 9, 2015·Frontiers in Microbiology·Jing WuLiming Liu
May 17, 2015·FEMS Yeast Research·Luis A Vale-Silva, Dominique Sanglard
Oct 22, 2016·Scientific Reports·Ludovic EnklerFabrice Jossinet
Jul 2, 2017·Applied and Environmental Microbiology·Xiaobao LinLiming Liu
Apr 9, 2014·Infection and Immunity·Matthias KretschmerJames W Kronstad
Sep 25, 2016·Applied and Environmental Microbiology·Dongni YanJian Chen
Nov 16, 2017·Molecular Oral Microbiology·M Rapala-KozikA Kozik
Mar 2, 2018·The Journal of Biological Chemistry·Mubashshir RasheedRupinder Kaur
Feb 2, 2019·Microorganisms·Kundan KumarRupinder Kaur
Feb 9, 2021·The Biochemical Journal·Bruna GonçalvesSónia Silva
Mar 20, 2015·Disease Models & Mechanisms·Sascha BrunkeDominique Ferrandon
Oct 18, 2019·Journal of Proteome Research·Mubashshir RasheedRupinder Kaur

❮ Previous
Next ❯

Related Concepts

Related Feeds

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.